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Prospec/VEGF Mouse (121 a.a.), Yeast/Vascular Endothelial Growth Factor (121 a.a.) Mouse Recombinant, Yeast/100µg/CYT-1125-100µg
Cataloguenumber
CYT-1125
Synonyms
VascularendothelialgrowthfactorA,VEGF-A,VascularpermeABIlityfactor,VPF,VEGF,MGC70609.
Introduction
Vascularendothelialgrowthfactorisanimportantsignalingproteininvolvedinbothangiogenesisandvasculogenesis.VEGFactivityhasbeenmostlystudiedoncellsofthevascularendothelium,althoughitdoeshaveeffectsonanumberofothercelltypes(e.g.stimulationmonocyte/macrophagemigration,neurons,cancercells,kidneyepithelialcells).VEGFmediatesincreasedvascularpermeability,inducesangiogenesis,vasculogenesisandendothelialcellgrowth,promotescellmigration,andinhibitsapoptosis.Invitro,VEGFhasbeenshowntostimulateendothelialcellmitogenesisandcellmigration.VEGFisalsoavasodilatorandincreasesmicrovascularpermeabilityandwasoriginallyreferredtoasvascularpermeabilityfactor.ElevatedlevelsofVEGFislinkedtoPOEMSsyndrome,alsoknownasCrow-Fukasesyndrome.MutationsinVEGFhavebeenassociatedwithproliferativeandnonproliferativediabeticretinopathy.
Description
VascularEndothelialGrowthFactor(121a.a.)MouseRecombinantproducedinyeastisadisulfide-linkedhomodimerconsistingof2x121aminoacidpolypeptidechains,havingamolecularmassofapproximately20.7kDaeach.VEGF(121a.a.)ispurifiedbyproprietarychromatographictechniques.
Source
Saccharomycescerevisiae
PhysicalAppearance
SterileFilteredWhitelyophilized(freeze-dried)powder.
Formulation
Lyophilizedfroma0.2μmfilteredconcentratedsolutioninPBS,pH7.4.
Solubility
ItisrecommendedtoreconstitutethelyophilizedVascularEndothelialGrowthFactor(121a.a.)insterile18MΩ-cmH2Onotlessthan100µg/ml,whichcanthenbefurtherdilutedtootheraqueoussolutions.
Stability
LyophilizedVEGF(121a.a.)althoughstableatroomtemperaturefor3weeks,shouldbestoreddesiccatedbelow-18°C.UponreconstitutionVascularEndothelialGrowthFactor(121a.a.)shouldbestoredat4°Cbetween2-7daysandforfutureusebelow-18°C.Pleasepreventfreeze-thawcycles.
Purity
Greaterthan95.0%asdeterminedby:(a)AnalysisbyRP-HPLC.(b)AnalysisbySDS-PAGE.
Aminoacidsequence
MAPTTEGEQKSHEVIKFMDVYQRSYCRPIETLVDIFQEYPDEIEYIFKPSCVPLMRCAGCCNDEALECVPTSESNITMQIMRIKPHQSQHIGEMSFLQHSRCECRPKKDRTKPEKCDKPRR.
BIOLOGicalActivity
TheactivitywasmeasuredinacellproliferationassayusingHUVEChumanumbilicalveinendothelialcellsandwasfoundtobe1-4ng/ml.
SafetyDataSheet
SDS
