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Prospec/Prolactin Chicken/Prolactin Chicken Recombinant/50µg/CYT-1064-50µg
Cataloguenumber
CYT-1064
Synonyms
Introduction
Prolactinisaneuroendocrinehormonesynthesizedprimarilybythepituitaryglandbutalsoavarietyofothercelltypesincludingtheplacenta,brainanduterus.Prolactinissecretedwheneating,nursing,mating,estrogentreatmentandduringovulation.Prolactin"sprimaryroleistopromoteandmaintainlactationbutalsoplaysaroleinbreastcancerdevelopment,regulationofreproductivefunctionandimmunoregulation.
Description
ProlactinChickenRecombinantproducedinE.Coliisasingle,non-glycosylatedpolypeptidechaincontaining199aminoacids+anadditionalAlaatn-terminalandhavingamolecularmassof~23kDa.
TheChickenProlactinproteinispurifiedbyproprietarychromatographictechniques.
Source
PhysicalAppearance
Formulation
Theproteinwaslyophilizedfromaconcentrated(1mg/ml)solutionwith0.02%NaHCO3.
Solubility
ItisrecommendedtoreconstitutethelyophilizedProlactininsterile0.4%NaHCO3pH-8.5,notlessthan100µg/ml,whichcanthenbefurtherdilutedtootheraqueoussolutions.
StABIlity
LyophilizedProlactinalthoughstableatroomtemperaturefor3weeks,shouldbestoreddesiccatedbelow-18°C.UponreconstitutionProlactinshouldbestoredat4°Cbetween2-7daysandforfutureusebelow-18°C.
Forlongtermstorageitisrecommendedtoaddacarrierprotein(0.1%HSAorBSA).
Pleasepreventfreeze-thawcycles.
Purity
Greaterthan95.0%asdeterminedbygelfiltrationanalysis.
Aminoacidsequence
Thea.a.sequenceofthefirst6N-terminala.awasfoundtobeAla-Leu-Pro-Ile-Cys-Pro.
BIOLOGicalActivity
ChickenProlactinwastestedforitsbiologicalfunctionalityin-vitrobyinducingproliferationofNb2cellsorBaf/3cellsthatwerestablytransfectedwithChickenProlactinreceptors.
SafetyDataSheet
SDS