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Prospec/Noggin Human, Sf9 Active/Noggin Human Recombinant, Sf9 Active/2µg/CYT-1119-2µg
Cataloguenumber
CYT-1119
Synonyms
SYM1,SYNS1,NOG.
Introduction
NogginwhichisencodedbytheNOGgene,bindsandinactivatesmembersofthetransforminggrowthfactor-beta(TGF-beta)superfamilysignalingproteins,suchasbonemorphogeneticprotein-4(BMP4).BydiffusingthroughextracellularmatricesmoreefficientlythanmembersoftheTGF-betasuperfamily,nogginmayplayandimportantroleincreatingmorphogenicgrADIents.Nogginappearstohavepleiotropiceffect,bothearlyindevelopmentaswellasinlaterstages.NogginwasoriginallyisolatedfromXenopusbasedonitsABIlitytorestorenormaldorsal-ventralbodyaxisinembryosthathadbeenartificiallyventralizedbyUVtreatment.Theresultsofthemouseknockoutofnogginsuggestthatitisinvolvedinnumerousdevelopmentalprocesses,suchasneuraltubefusionandjointformation.ThereareseveraldominanthumanNOGmutationsinunrelatedfamilieswithproximalsymphalangism(SYM1)andmultiplesynostosessyndrome(SYNS1).AllNOGmutationsalteredevolutionarilyconservedaminoacidresidues.TheaminoacidsequenceofhumannogginishighlyhomologoustothatofXenopus,ratandmouse.
Description
NogginproducedinSf9Baculoviruscellsisaglycosylatedhomodimercontaining205aminoacidsandhavingamolecularmassof47.9kDaundernon-reducingconditions.(MolecularsizeonSDS-PAGEwillappearatapproximately50-80kDa).Nogginispurifiedbyproprietarychromatographictechniques.
Source
Sf9,Baculoviruscells.
PhysicalAppearance
SterileFilteredWhitelyophilized(freeze-dried)powder.
Formulation
Lyophilizedfroma0.2μmfilteredconcentratedsolutioninPBS,pH7.4and0.02%Tween-20and5%trehalose.
Solubility
ItisrecommendedtoreconstitutethelyophilizedNoggininsterile18MΩ-cmH2Onotlessthan100µg/ml,whichcanthenbefurtherdilutedtootheraqueoussolutions.
Stability
LyophilizedNogginalthoughstableatroomtemperaturefor3weeks,shouldbestoreddesiccatedbelow-18°C.UponreconstitutionNogginshouldbestoredat4°Cbetween2-7daysandforfutureusebelow-18°C.Pleasepreventfreeze-thawcycles.
Purity
Greaterthan95.0%asdeterminedby:(a)AnalysisbyRP-HPLC(b)AnalysisbySDS-PAGE.
Aminoacidsequence
QHYLHIRPAPSDNLPLVDLIEHPDPIFDPKEKDLNETLLRSLLGGHYDPGFMATSPPEDRPGGGGGAAGGAEDLAELDQLLRQRPSGAMPSEIKGLEFSEGLAQGKKQRLSKKLRRKLQMWLWSQTFCPVLYAWNDLGSRFWPRYVKVGSCFSKRSCSVPEGMVCKPSKSVHLTVLRWRCQRRGGQRCGWIPIQYPIISECKCSC.
BIOLOGicalActivity
MeasuredbyitsabilitytoinhibitBMP-4-inducedalkalinephosphataseproductionbyATDC5mousechondrogeniccellsanswasfounttobe0.04‑0.2μg/mLinthepresenceof50ng/mLofRecombinantHumanBMP‑4.
SafetyDataSheet
SDS
