Cataloguenumber
CYT-1076
Synonyms
Insulin,Insulin-DependentDiabetesMellitus2,Preproinsulin,Proinsulin,MODY10,IDDM1,IDDM2,IDDM,ILPR,IRDN.
Introduction
Insulindecreasesbloodglucoseconcentration.itincreasescellpermeABIlitytomonosaccharides,aminoacidsandfattyacids.itacceleratesglycolysis,thepentosephosphatecycle,andglycogensynthesisinliver.
Description
InsulinRecombinantproducedinE.Coliisasingle,non-glycosylatedpolypeptidechaincontaining109aminoacids(25-110a.a)andhavingamolecularmassof11.8kDa.Insulinisfusedtoa23aminoacidHis-tagatN-terminus&purifiedbyproprietarychromatographictechniques.
Source
PhysicalAppearance
Formulation
Insulinproteinsolution(0.5mg/ml)containingPhosphate-BufferedSaline(pH7.4)and10%Glycerol.
Stability
Purity
Greaterthan95.0%asdeterminedbySDS-PAGE.
BIOLOGicalActivity
MeasuredinacellproliferationassayusingMCF7humanbreastcancercell.TheED50forthiseffectislessorequalto4ug/ml.
Aminoacidsequence
MGSSHHHHHHSSGLVPRGSHMGSFVNQHLCGSHLVEALYLVCGERGFFYTPKTRREAEDLQVGQVELGGGPGAGSLQPLALEGSLQKRGIVEQCCTSICSLYQLENYCN
SafetyDataSheet
SDS
Usage
Prospec"sproductsarefurnishedforLABORATORYRESEARCHUSEONLY.Theproductmaynotbeusedasdrugs,agriculturalorpesticidalproducts,foodadditivesorhouseholdchemicals.