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Prospec/IGF1 Human, V44M/Insulin Like Growth Factor-1, Mutant V44M Human Recombinant/100µg/CYT-1088-100µg

Cataloguenumber
CYT-1088
Synonyms
Introduction
Thesomatomedins,orinsulin-likegrowthfactors(IGFs),compriseafamilyofpeptidesthattakespartinmammaliangrowthanddevelopment.IGF1mediatesvariousgrowth-promotingeffectsofgrowthhormone(GH;MIM139250).Earlystudiesshowedthatgrowthhormonedidnotdirectlystimulatetheincorporationofsulfateintocartilage,butratheractedthroughaserumfactor,termed"sulfationfactor,"whichlaterbecameknownas"somatomedin"(Daughadayetal.,1972).3mainsomatomedinshavebeencharacterized:somatomedinC(IGF1),somatomedinA(IGF2;MIM147470),andsomatomedinB(MIM193190)(Rotwein,1986;Rosenfeld,2003).
Description
IGF1V44MHumanRecombinantproducedinE.Coliisasingle,non-glycosylatedpolypeptidechaincontaining70aminoacidsandhavingamolecularmassofApproximately7.7kDa.
TheIGF1V44Mispurifiedbyproprietarychromatographictechniques.
Source
PhysicalAppearance
Formulation
IGF1V44MLyophilizedfroma0.2µmfilteredconcentratedsolutioninPBS,pH7.0.
Solubility
ItisrecommendedtoreconstitutethelyophilizedIGF1V44Minsterile18MΩ-cmH2Onotlessthan100µg/ml,whichcanthenbefurtherdilutedtootheraqueoussolutions.
StABIlity
Storeat4°Cifentirevialwillbeusedwithin2-4weeks.
Store,frozenat-20°Cforlongerperiodsoftime.Forlongtermstorageitisrecommendedtoaddacarrierprotein(0.1%HSAorBSA).Avoidmultiplefreeze-thawcycles.
Purity
Greaterthan95.0%asdeterminedbySDS-PAGE.
Aminoacidsequence
GPETLCGAELVDALQFVCGDRGFYFNKPTGYGSSSRRAPQTGIMDECCFRSCDLRRLEMY.
SafetyDataSheet
SDS