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Prospec/FGF17 Mouse/Fibroblast Growth Factor 17 Mouse Recombinant/20µg/CYT-1123-20µg
Cataloguenumber
CYT-1123
Synonyms
Fibroblastgrowthfactor17,FGF-17,FGF17,FGF-13,HH20.
Introduction
FibroblastGrowthFactor17(FGF17)isapartofthefibroblastgrowthfactorfamily.FGFfamilymembershavebroadmitogenicandcellsurvivalactivities,andareinvolvedinvariousBIOLOGicalprocessesincludingmorphogenesis,embryonicdevelopmentcellgrowth,,tissuerepair,tumorgrowthandinvasion.TheFGF17geneishighlyexpressedinthecerebellumandcortex.ThemousehomologoftheFGF17geneislocalizedtospecificsitesinthemidlinestructuresoftheforebrain,themidbrain-hindbrainjunction,developingskeletonanddevelopingarteries,suggestingapartincentralnervoussystem,boneandvasculardevelopment.
Description
FibroblastGrowthFactor17MouseRecombinantproducedinE.Coliisanon-glycosylatedpolypeptidechaincontaining194aminoacidandhavingamolecularmassofapproximately22.5kDa.FGF17ispurifiedbyproprietarychromatographictechniques.
Source
EscherichiaColi.
PhysicalAppearance
SterileFilteredWhitelyophilized(freeze-dried)powder.
Formulation
Lyophilizedfroma0.2μmfilteredconcentratedsolutionin20mMTris-HCl,pH8.0,0.02%Tween-20and700mMNaCl.
Solubility
ItisrecommendedtoreconstitutethelyophilizedFibroblastGrowthFactor17insterilePBSnotlessthan100µg/ml,whichcanthenbefurtherdilutedtootheraqueoussolutions.
StABIlity
LyophilizedFGF17althoughstableatroomtemperaturefor3weeks,shouldbestoreddesiccatedbelow-18°C.UponreconstitutionFibroblastGrowthFactor17shouldbestoredat4°Cbetween2-7daysandforfutureusebelow-18°C.
Pleasepreventfreeze-thawcycles.
Purity
Greaterthan98.0%asdeterminedby:(a)AnalysisbyRP-HPLC.(b)AnalysisbySDS-PAGE.
Aminoacidsequence
TQGENHPSPNFNQYVRDQGAMTDQLSRRQIREYQLYSRTSGKHVQVTGRRISATAEDGNKFAKLIVETDTFGSRVRIKGAESEKYICMNKRGKLIGKPSGKSKDCVFTEIVLENNYTAFQNARHEGWFMAFTRQGRPRQASRSRQNQREAHFIKRLYQGQLPFPNHAERQKQFEFVGSAPTRRTKRTRRPQSQT.
BiologicalActivity
TheED50asdeterminedbyacellproliferationassayusingmurinebalb/c3T3cellsis<10ng/ml,correspondingtoaspecificactivityof>1.0×105IU/mginthepresenceof10μg/mlofheparin.
SafetyDataSheet
SDS