Cataloguenumber
CYT-498
Synonyms
GH1,GH,GHN,GH-N,hGH-N,PituitaryGH,GH1.
Introduction
GHisamemberoftheprolactinfamilyofhormoneswhichplayanimportantroleingrowthcontrol.Thegene,alongwithfourotherrelatedgenes,islocatedattheGHlocusonchromosome17wheretheyareinterspersedinthesametranscriptionalorientation;anarrangementwhichisthoughttohaveevolvedbyaseriesofgeneduplications.Thefivegenessharearemarkablyhighdegreeofsequenceidentity.AlternativesplicinggeneratesadditionalisoformsofeachofthefiveGHs,leADIngtofurtherdiversityandpotentialforspecialization.ThisparticularfamilymemberisexpressedinthepituitarybutnotinplacentaltissueasisthecasefortheotherfourgenesintheGHlocus.MutationsinordeletionsofthegeneleadtoGHdeficiencyandshortstature.
Description
GHDenisRecombinantproducedinE.Coliisasingle,non-glycosylated,polypeptidechaincontaining190aminoacidsandhavingamolecularmassof21810Dalton.GHispurifiedbyproprietarychromatographictechniques.
Source
PhysicalAppearance
Formulation
Solubility
ItisrecommendedtoreconstitutethelyophilizedGHDenisinsterile18MΩ-cmH2Onotlessthan100µg/ml,whichcanthenbefurtherdilutedtootheraqueoussolutions.
StABIlity
LyophilizedGHDenisalthoughstableatroomtemperaturefor3weeks,shouldbestoreddesiccatedbelow-18°C.UponreconstitutionGHDenisshouldbestoredat4°Cbetween2-7daysandforfutureusebelow-18°C.Forlongtermstorageitisrecommendedtoaddacarrierprotein(0.1%HSAorBSA).Pleasepreventfreeze-thawcycles.
Purity
SafetyDataSheet
SDS