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Prospec/FGF1 Human, 154 a.a./Fibroblast Growth Factor-acidic (154 a.a.) Human Recombinant/50µg/CYT-1112-50µg

Cataloguenumber
CYT-1112
Synonyms
HBGF-1,ECGF-beta,FIBP,FGFIBP,FIBP-1,ECGF,ECGFA,GLIO703,FGF1,FGF-a.
Introduction
Acidicfibroblastgrowthfactorisamemberofthefibroblastgrowthfactor(FGF)family.FGFfamilymemberspossessbroadmitogenicandcellsurvivalactivities,andareinvolvedinavarietyofBIOLOGicalprocesses,includingembryonicdevelopment,cellgrowth,morphogenesis,tissuerepair,tumorgrowthandinvasion.FGF1functionsasamodifierofendothelialcellmigrationandproliferation,aswellasanangiogenicfactor.FGF1actsasamitogenforavarietyofmesoderm-andneuroectoderm-derivedcellsinvitro,thusisthoughttobeinvolvedinorganogenesis.Threealternativelysplicedvariantsencodingdifferentisoformshavebeendescribed.Thebindinggrowthfactorsareangiogenicagentsinvivoandarepotentmitogensforavarietyofcelltypesinvitro.Therearedifferencesinthetissuedistributionandconcentrationofthese2growthfactors.
Description
FibroblastGrowthFactor-acidicHumanRecombinantproducedinE.Coliisasingle,non-glycosylatedpolypeptidechaincontaining154aminoacidsandhavingamolecularmassof17.3kDa.TheFGF1ispurifiedbyproprietarychromatographictechniques.
Source
EscherichiaColi.
PhysicalAppearance
SterileFilteredWhitelyophilized(freeze-dried)powder.
Formulation
Lyophilizedfroma0.2μmfilteredconcentratedsolutioninPBS,pH7.4,with0.5mMDTT,2mMEDTA,and5%Trehalose.
Solubility
ItisrecommendedtoreconstitutethelyophilizedFibroblastGrowthFactor-acidicRecombinantinsterile18MΩ-cmH2Onotlessthan100µg/ml,whichcanthenbefurtherdilutedtootheraqueoussolutions.
StABIlity
LyophilizedFGF1althoughstableatroomtemperaturefor3weeks,shouldbestoreddesiccatedbelow-18°C.UponreconstitutionFibroblastGrowthFactor-acidicshouldbestoredat4°Cbetween2-7daysandforfutureusebelow-18°C.
Pleasepreventfreeze-thawcycles.
Aminoacidsequence
AEGEITTFTALTEKFNLPPGNYKKPKLLYCSNGGHFLRILPDGTVDGTRDRSDQHIQLQLSAESVGEVYIKSTETGQYLAMDTDGLLYGSQTPNEECLFLERLEENHYNTYISKKHAEKNWFVGLKKNGSCKRGPRTHYGQKAILFLPLPVSSD.
Purity
Greaterthan97.0%asdeterminedby:
(a)AnalysisbyRP-HPLC.
(b)AnalysisbySDS-PAGE.
BiologicalActivity
TheED50asdeterminedbyacellproliferationassayusingmurinebalb/c3T3cellsis<than0.5ng/ml,correspondingtoaspecificactivityof>2.0×106IU/mginthepresenceof10µg/mlHeparin.
SafetyDataSheet
SDS