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Prospec/EGF (1-51), Human/Epidermal Growth Factor (1-51 a.a.)Human Recombinant/100µg/CYT-1115-100µg
Cataloguenumber
CYT-1115
Synonyms
Urogastrone,URG,EGF.
Introduction
Epidermalgrowthfactorhasaprofoundeffectonthedifferentiationofspecificcellsinvivoandisapotentmitogenicfactorforavarietyofculturedcellsofbothectodermalandmesodermalorigin.TheEGFprecursorisbelievedtoexistasamembrane-boundmoleculewhichisproteolyticallycleavedtogeneratethe53-aminoacidpeptidehormonethatstimulatescellstodivide.EGFstimulatesthegrowthofseveralepidermalandepithelialtissuesinvivoandinvitroandofsomefibroblastsincellculture.
Description
EpidermalGrowthFactor(1-51a.a.)HumanRecombinantproducedinyeastisasingle,glycosylatedpolypeptidechaincontaining51aminoacidsandhavingamolecularmassof6.0kDa.TheEGFispurifiedbyproprietarychromatographictechniques.
Source
Saccharomycescerevisiae
PhysicalAppearance
SterileFilteredWhitelyophilized(freeze-dried)powder.
Formulation
Lyophilizedfroma0.2μmfilteredconcentratedsolutioninPBS,pH7.4.
Solubility
ItisrecommendedtoreconstitutethelyophilizedEpidermalGrowthFactorinsterile18MΩ-cmH2Onotlessthan100µg/ml,whichcanthenbefurtherdilutedtootheraqueoussolutions.
StABIlity
LyophilizedEGFalthoughstableatroomtemperaturefor3weeks,shouldbestoreddesiccatedbelow-18°C.UponreconstitutionEpidermalGrowthFactorshouldbestoredat4°Cbetween2-7daysandforfutureusebelow-18°C.Pleasepreventfreeze-thawcycles.
Purity
Greaterthan98.0%asdeterminedby:(a)AnalysisbyRP-HPLC.(b)AnalysisbySDS-PAGE.
Aminoacidsequence
NSDSECPLSHDGYCLHDGVCMYIEALDKYACNCVVGYIGERCQYRDLKWWE.
BIOLOGicalActivity
TheED50isdeterminedbyacellproliferationassayusingmurineBalb/c3T3cellsandis<than0.1ng/ml,correspondingtoaspecificactivityof>1.0×107IU/mg.
SafetyDataSheet
SDS