Cataloguenumber
CYT-1095
Synonyms
CSF-2,MGI-1GM,GM-CSF,Pluripoietin-alpha,Molgramostin,Sargramostim.
Introduction
GMCSFisacytokinethatcontrolstheproduction,differentiation,andfunctionofgranulocytesandmacrophages.TheactiveformofGMCSFisfoundextracellularlyasahomodimer.GMCSFhasbeenlocalizedtoaclusterofrelatedgenesatchromosomeregion5q31,whichisknowntobeassociatedwithinterstitialdeletionsinthe5q-syndromeandacutemyelogenousleukemia.Othergenesintheclusterincludethoseencodinginterleukins4,5,and13.GM-CSFstimulatesthegrowthanddifferentiationofhematopoieticprecursorcellsfromvariouslineages,includinggranulocytes,macrophages,eosinophilsanderythrocytes.
Description
GranulocyteMacrophage-ColonyStimulatingFactorPorcineRecombinantproducedinE.coliisanon-glycosylatedmonomerchaincontaining128aminoacidsandhavingamolecularmassof14.5kDa.
GMCSFispurifiedbyproprietarychromatographictechniques.
Source
EscherichiaColi.
PhysicalAppearance
Formulation
Theproteinwaslyophilizedfromasterile(0.2µm)filteredsolutioncontaining10mMsodiumphosphate,pH7.5.
Solubility
ItisrecommendedtoreconstitutethelyophilizedGMCSFinsterile18MΩ-cmH2Onotlessthan100µg/ml,whichcanthenbefurtherdilutedtootheraqueoussolutions.
StABIlity
LyophilizedGMCSFalthoughstableatroomtemperaturefor3weeks,shouldbestoreddesiccatedbelow-18°C.UponreconstitutionGMCSFshouldbestoredat4°Cbetween2-7daysandforfutureusebelow-18°C.
Pleasepreventfreeze-thawcycles.
Aminoacidsequence
MAPTRPPSPVTRPWQHVDAIKEALSLLNNSNDTAAVMNETVDVVCEMFDPQEPTCVQTRLNLYKQGLRGSLTRLKSPLTLLAKHYEQHCPLTEETSCETQSITFKSFKDSLNKFLFTIPFDCWGPVKK.
Purity
Greaterthan95.0%asdeterminedbySDS-PAGE.
BIOLOGicalActivity
TheED50,asdeterminedbyTF-1cellproliferationis4.52ng/mlcorrespondingtoaspecificactivitywhichis2.2x10^5units/mg.
SafetyDataSheet
SDS