Cataloguenumber
CYT-1121
Synonyms
CSF3,MGI-1G,GM-CSFbeta,Pluripoietin,G-CSF,GCSF.
Introduction
GCSFisacytokinethatcontrolstheproduction,differentiation,andfunctionofgranulocytes.Theactiveproteinisfoundextracellularly.3transcriptvariantsencoding3differentisoformshavebeenfoundfortheGCSFgene.Granulocyte/macrophagecolony-stimulatingfactorsarecytokinesthattakepartinhematopoiesisbycontrollingtheproduction,differentiation,andfunctionof2relatedwhitecellpopulationsoftheblood,thegranulocytesandthemonocytes-macrophages.Thiscsfinducesgranulocytes.
Description
GranulocyteColonyStimulatingRhesusMacaqueRecombinantproducedinE.Coliisanon-glycosylatedpolypeptidechaincontaining174aminoacidsandhavingamolecularmassofapproximately18.9kDa.GCSFispurifiedbyproprietarychromatographictechniques.
Source
EscherichiaColi.
PhysicalAppearance
SterileFilteredWhitelyophilized(freeze-dried)powder.
Formulation
Lyophilizedfroma0.2μmfilteredconcentratedsolutioninPBS,pH7.4.
Solubility
ItisrecommendedtoreconstitutethelyophilizedGranulocyteColonyStimulatingRhesusMacaqueinsterile18MΩ-cmH2Onotlessthan100µg/ml,whichcanthenbefurtherdilutedtootheraqueoussolutions.
StABIlity
LyophilizedGCSFalthoughstableatroomtemperaturefor3weeks,shouldbestoreddesiccatedbelow-18°C.UponreconstitutionGranulocyteColonyStimulatingRhesusMacaqueshouldbestoredat4°Cbetween2-7daysandforfutureusebelow-18°C.Pleasepreventfreeze-thawcycles.
Purity
Greaterthan98.0%asdeterminedby:(a)AnalysisbyRP-HPLC.(b)AnalysisbySDS-PAGE.
Aminoacidsequence
TPLGPASSLPQSFLLKCLEQVRKIQGDGAALQEKLCATYKLCHPEELVLLRHSLGIPWAPLSSCPSQALQLTGCLSQLHSSLFLYQGLLQALEGISPELSPTLDTLQLDIADFATTIWQQMEDLGMAPALQPTQGAMPAFTSAFQRRAGGVLVASHLQRFLELAYRVLRHLAQS.
BIOLOGicalActivity
TheED50asdeterminedbyacellproliferationassayusingmurineNFS-60cellsis<0.05ng/ml,correspondingtoaspecificactivityof>2.0×107IU/mg.
SafetyDataSheet
SDS