Cataloguenumber
CYT-1044
Synonyms
ColonyStimulatingFactor2ReceptorAlphaSubunit,ColonyStimulatingFactor2Receptor,Alpha,Low-Affinity(Granulocyte-Macrophage),Alpha-GM-CSFReceptor,GM-CSF-R-Alpha,CD116Antigen,GMCSFR-Alpha,GMR-Alpha,CDw116,CSF2RY,CSF2R,Granulocyte-MacrophageColony-StimulatingFactorReceptorSubunitAlpha,Granulocyte-MacrophageColony-StimulatingFactorReceptorAlphaChain,GM-CSFReceptorAlphaSubunit,AlphaGMR,CSF2RAX,CSF2RAY,CSF2RX,GMCSFR,CD116,SMDP4,GMR.
Introduction
GM-CSFReceptorAlpha(CSF2RA)isthealphasubunitoftheheterodimericreceptorforcolonystimulatingfactor2,acytokinewhichcontrolstheproduction,differentiation,andfunctionofgranulocytesandmacrophages.CSFR2isalsoamemberofthecytokinefamilyofreceptors.Inaddition,thisgeneisfoundinthepseudoautosomalregion(PAR)oftheXandYchromosomes.Multipletranscriptvariantsencodingvariousisoformshavebeenfoundforthisgene,whilesomeoftheisoformsbeingmembrane-boundandothersbeingsoluble.DiseasesassociatedwithCSF2RAincludesurfactantmetabolismdysfunction,pulmonary4,andcsf2ra-relatedpulmonarysurfactantmetabolismdysfunction.
Description
CSF2RAproducedinSf9Baculoviruscellsisasingle,glycosylatedpolypeptidechaincontaining310aminoacids(20-320a.a.)andhavingamolecularmassof35.9kDa.(MolecularsizeonSDS-PAGEwillappearatapproximately40-57kDa).CSF2RAisexpressedwitha9aminoacidHistagatC-Terminusandpurifiedbyproprietarychromatographictechniques.
Source
PhysicalAppearance
Formulation
CSF2RAproteinsolution(0.5mg/ml)containsPhosphateBufferedSaline(pH7.4)and10%glycerol.
StABIlity
Purity
Greaterthan90.0%asdeterminedbySDS-PAGE.
BIOLOGicalActivity
MeasuredbyitsabilitytoinhibitGM-CSFdependentproliferationofTF1humanerythroleukemiccells.TheED50forthiseffectislessorequalto10ug/mlinthepresenceof0.5ng/mlGM-CSF.
Aminoacidsequence
ADPLIPEKSDLRTVAPASSLNVRFDSRTMNLSWDCQENTTFSKCFLTDKKNRVVEPRLSNNECSCTFREICLHEGVTFEVHVNTSQRGFQQKLLYPNSGREGTAAQNFSCFIYNADLMNCTWARGPTAPRDVQYFLYIRNSKRRREIRCPYYIQDSGTHVGCHLDNLSGLTSRNYFLVNGTSREIGIQFFDSLLDTKKIERFNPPSNVTVRCNTTHCLVRWKQPRTYQKLSYLDFQYQLDVHRKNTQPGTENLLINVSGDLENRYNFPSSEPRAKHSVKIRAADVRILNWSSWSEAIEFGSDDGHHHHHH
SafetyDataSheet
SDS