Cataloguenumber
CYT-1132
Synonyms
SerpinA12precursor,VisceralADIpose-specificserpin,Visceraladiposetissue-derivedserineproteaseinhibitor,Vaspin,OL-64,SERPINA12,Serine(orcysteine)proteinaseinhibitor,cladeA,antitrypsin,alpha-1antiproteinase.
Introduction
Vaspin(visceraladipose-specificSERPIN)isanewlyidentifiedadipokine,whichisamemberofserineproteaseinhibitorfamily.Vaspinisalsoauniqueinsulinsensitizingadipocytokineinobesity.ArecentpublicationindicatesthatinductionofhumanvaspinmRNAexpressioninadiposetissueisregulatedinafatdepot-specificmannerandcouldbeassociatedwithparametersofobesity,insulinresistance,andglucosemetabolism.
Description
VaspinHumanRecombinantproducedinE.Coliisasingle,non-glycosylatedpolypeptidechaincontaining394aminoacidsandhavingamolecularmassof45.1kDa.Vaspinispurifiedbyproprietarychromatographictechniques.
Source
EscherichiaColi.
PhysicalAppearance
SterileFilteredWhitelyophilized(freeze-dried)powder.
Formulation
Lyophilizedfroma0.2μmfilteredconcentratedsolutionin20mMTris-HCl,pH8.0,150mMNaCland0.02%Tween-20.
Solubility
ItisrecommendedtoreconstitutethelyophilizedVaspininsterile18MΩ-cmH2Onotlessthan100µg/ml,whichcanthenbefurtherdilutedtootheraqueoussolutions.
StABIlity
LyophilizedVaspinalthoughstableatroomtemperaturefor3weeks,shouldbestoreddesiccatedbelow-18°C.UponreconstitutionVaspinshouldbestoredat4°Cbetween2-7daysandforfutureusebelow-18°C.Pleasepreventfreeze-thawcycles.
Purity
Greaterthan98.0%asdeterminedby:(a)AnalysisbyRP-HPLC.(b)AnalysisbySDS-PAGE.
Aminoacidsequence
LKPSFSPRNYKALSEVQGWKQRMAAKELARQNMDLGFKLLKKLAFYNPGRNIFLSPLSISTAFSMLCLGAQDSTLDEIKQGFNFRKMPEKDLHEGFHYIIHELTQKTQDLKLSIGNTLFIDQRLQPQRKFLEDAKNFYSAETILTNFQNLEMAQKQINDFISQKTHGKINNLIENIDPGTVMLLANYIFFRARWKHEFDPNVTKEEDFFLEKNSSVKVPMMFRSGIYQVGYDDKLSCTILEIPYQKNITAIFILPDEGKLKHLEKGLQVDTFSRWKTLLSRRVVDVSVPRLHMTGTFDLKKTLSYIGVSKIFEEHGDLTKIAPHRSLKVGEAVHKAELKMDERGTEGAAGTGAQTLPMETPLVVKIDKPYLLLIYSEKIPSVLFLGKIVNPIGK.
SafetyDataSheet
SDS
