Cataloguenumber
CYT-318
Synonyms
PBEF,Pre-Bcellcolony-enhancingfactor,NicotinamidephosphoribosyltransferaseNAmPRTase,Nampt,MGC117256,DKFZP666B131,1110035O14Rik.
Introduction
ExcessADIposityisthemostimportantriskinthedevelopmentoftype2diabetesmellitus(T2DM).Adiposetissueproducesseveralproteins(adipocytokines)suchasleptin,adiponectin,resistin,tumornecrosisfactor-a,andIL-6,thatmodulatesensitivityandappeartoplayanimportantroleinthepathogenesis,diabetes,dyslipidemia,inflammation,andatherosclerosis.Visfatin,alsoknownaspre-Bcellcolony-enhancingfactor(PBEF),isacytokinethatishighlyexpressedinvisceralfatandwasoriginallyisolatedasasecretedfactorthatsynergizeswithIL-7andstemcellfactorstopromotethegrowthofBcellprecursors.Visfatinhomologshavebeenidentifiedincarp,invertebratemollusks,andbacteria,aswellasinvertebrates,includinghumansandthemouse.Ithasbeenpostulatedtoplayaroleininnateimmunity.Visfatinexertsmimeticeffectsthataredose-dependentandquantitativelysimilartostimulatingmuscleandadipocyteglucosetransport,andininhibitinghepatocyteglucoseproduction.Intravenousinjectionofrecombinantvisfatininmicedecreasedplasmaglucoseinadose-dependentfashion.Inkeepingwithitsmimeticeffects,visfatinwasaseffectiveinreducinghyperglycemiaindeficientdiabeticmice.Visfatinwasalsofoundtobeboundtoandactivatereceptor,causingreceptorphosphorylationandtheactivationofdownstreamsignalingmolecules.However,visfatindidnotcompeteforbindingtothereceptor,indicatingthatthetwoproteinswererecognizedbydifferentregionsofthereceptor.Thus,visfatinmightplayaroleinglucosehomeostasisanddysregulationinbiosynthesisorsignaltransduction,andmightcontributetothepathogenesisofdiabetes.
Description
VisfatinHumanRecombinantproducedinE.Coliisasingle,non-glycosylated,polypeptidechaincontaining466aminoacids.Thetotalmolecularmassis52.6kDa(calculated).TheVisfatinispurifiedbyFlag-affinitychromatography.
Source
EscherichiaColi.
PhysicalAppearance
FilteredWhitelyophilized(freeze-dried)powder.
Formulation
Visfatinwaslyophilizedwithnoadditives.
Solubility
Centrifugevialbeforeopening.Whenreconstitutingtheproduct,gentlypipetandwashdownthesidesofthevialtoensurefullrecoveryoftheproteinintosolution.Itisrecommendedtoreconstitutethelyophilizedproductwith20mMHClataconcentrationof0.1mg/mL,whichcanbefurtherdilutedintootheraqueoussolutions.Waitseveralminutesforfullreconstitutionandsolubility.
LyophilizedVisfatinalthoughstableatroomtemperaturefor3weeks,shouldbestoreddesiccatedbelow-18°C.UponreconstitutionVisfatinshouldbestoredat4°Cbetween2-7daysandforfutureusebelow-18°C.Forlongtermstorageitisrecommendedtoaddacarrierprotein(0.1%HSAorBSA).Pleasepreventfreeze-thawcycles.
Purity
Greaterthan90.0%asdeterminedbySDS-PAGE.
Aminoacidsequence
MPPNTSKVYSYFECREKKTENSKLRKVKYEETVFYGLQYILNKYLKGKVVTKEKIQEAKDVYKEHFQDDVFNEKGWNYILEKYDGHLPIEIKAVPEGFVIPRGNVLFTVENTDPECYWLTNWIETILVQSWYPITVATNSREQKKILAKYLLETSGNLDGLEYKLHDFGYRGVSSQETAGIGASAHLVNFKGTDTVAGLALIKKYYGTKDPVPGYSVPAAEHSTITAWGKDHEKDAFEHIVTQFSSVPVSVVSDSYDIYNACEKIWGEDLRHLIVSRSTQAPLIIRPDSGNPLDTVLKVLEILGKKFPVTENSKGYKLLPPYLRVIQGDGVDINTLQEIVEGMKQKMWSIENIAFGSGGGLLQKLTRDLLNCSFKCSYVVTNGLGINVFKDPVADPNKRSKKGRLSLHRTPAGNFVTLEEGKGDLEEYGQDLLHTVFKNGKVTKSYSFDEIRKNAQLNIELEAAHH.
TheactivityisdeterminedbyitsabilitytoinduceIL-6,IL-1betaandTNFalphaproductionfromhumanPBMCsat100ng/ml.
SafetyDataSheet
Usage
Prospec"sproductsarefurnishedforLABORATORYRESEARCHUSEONLY.Theproductmaynotbeusedasdrugs,agriculturalorpesticidalproducts,foodadditivesorhouseholdchemicals.