
Cataloguenumber
CYT-699
Synonyms
Introduction
Description
TNFSF12HumanRecombinant(94-249a.a.)producedinE.Coliisasingle,non-glycosylated,polypeptidechaincontaining156aminoacidsandhavingatotalmolecularmassof18kDa.
TheTNFSF12isfusedwithan8aminoacidshistagatN-terminal(M-HHHHHH-R,total164a.a.)andpurifiedbyproprietarychromatographictechniques.
Source
Escherichiacoli.
PhysicalAppearance
Formulation
Lyophilizedfroma0.2μmfilteredsolutioninPBS.
Solubility
ItisrecommendedtoreconstitutethelyophilizedTNFSF12insterile18MΩ-cmH2Onotlessthan100µg/ml,whichcanthenbefurtherdilutedtootheraqueoussolutions.
StABIlity
LyophilizedTNFSF12althoughstableatroomtemperaturefor3weeks,shouldbestoreddesiccatedbelow-18°C.UponreconstitutionTWEAKshouldbestoredat4°Cbetween2-7daysandforfutureusebelow-18°C.
Forlongtermstorageitisrecommendedtoaddacarrierprotein(0.1%HSAorBSA).
Pleasepreventfreeze-thawcycles.
Aminoacidsequence
MHHHHHHRSAPKGRKTRARRAIAAHYEVHPRPGQDGAQAGVDGTVSGWEEARINSSSPLRYNRQIGEFIVTRAGLYYLYCQVHFDEGKAVYLKLDLLVDGVLALRCLEEFSATAASSLGPQLRLCQVSGLLALRPGSSLRIRTLPWAHLKAAPFLTYFGLFQVH.
Purity
Greaterthan95%asdeterminedbySDS-PAGE.
BIOLOGicalActivity
TheED50,asmeasuredinaproliferationassayusingHUVECs,islessthan8ng/ml.
SafetyDataSheet
SDS