
Cataloguenumber
CYT-1045
Synonyms
Tumornecrosisfactorreceptorsuperfamilymember10D,CD264,DCR2,TRAIL-R4,TRAILR4,TRUNDD,Decoyreceptor2,TNF-relatedapoptosis-inducingligandreceptor4,TRAILreceptor4,TRAILreceptorwithatruncateddeathdomain.
Introduction
TRAILReceptor-4HumanRecombinantorTNFRSF10D,ispartoftheTNF-receptorsuperfamily.TNFRSF10Dhasatruncatedcytoplasmicdeathdomai,anextracellularTRAIL-bindingdomainandatransmembranedomain.TheproteincanpreventfromTRAIL-mediatedapoptosisoncellswithTRAILR1and/orTRAILR2.
Description
TNFRSF10DproducedinSf9Baculoviruscellsisasingle,glycosylatedpolypeptidechaincontaining395aminoacids(56-211a.a.)andhavingamolecularmassof73.8kDa.(MolecularsizeonSDS-PAGEwillappearatapproximately40-57kDa).TNFRSF10Disexpressedwitha239aminoacidhIgG-HistagatC-Terminusandpurifiedbyproprietarychromatographictechniques.
Source
PhysicalAppearance
Formulation
TNFRSF10Dproteinsolution(0.5mg/ml)containsphosphatebufferedsaline(pH7.4)and10%glycerol.
StABIlity
Purity
Greaterthan90.0%asdeterminedbySDS-PAGE.
BIOLOGicalActivity
MeasuredinaneutralizingassayusingJurkathumanTlymphocyte.TheED50forthiseffectislessorequalto10ng/mlinthepresenceof2ng/mlTRAIL.
Aminoacidsequence
ATIPRQDEVPQQTVAPQQQRRSLKEEECPAGSHRSEYTGACNPCTEGVDYTIASNNLPSCLLCTVCKSGQTNKSSCTTTRDTVCQCEKGSFQDKNSPEMCRTCRTGCPRGMVKVSNCTPRSDIKCKNESAASSTGKTPAAEETVTTILGMLASPYHVEPKSCDKTHTCPPCPAPELLGGPSVFLFPPKPKDTLMISRTPEVTCVVVDVSHEDPEVKFNWYVDGVEVHNAKTKPREEQYNSTYRVVSVLTVLHQDWLNGKEYKCKVSNKALPAPIEKTISKAKGQPREPQVYTLPPSRDELTKNQVSLTCLVKGFYPSDIAVEWESNGQPENNYKTTPPVLDSDGSFFLYSKLTVDKSRWQQGNVFSCSVMHEALHNHYTQKSLSLSPGKHHHHHH.
SafetyDataSheet
SDS