Cataloguenumber
CYT-1057
Synonyms
TumorNecrosisFactor-alpha,TNFaHis,Cachectin,TNF-alpha,Tumornecrosisfactorligandsuperfamilymember2,TNF-a,N-terminalfragment,NTF,Intracellulardomain1,Intracellulardomain2,ICD2,C-domain1,C-domain2,Tumornecrosisfactor,solubleform,Tnfa,Tnfsf2,RATTNF,Tnfa.
Introduction
Tumornecrosisfactorisacytokineinvolvedinsystemicinflammationandisamemberofagroupofcytokinesthatallstimulatetheacutephasereaction.TNFismainlysecretedbymacrophages.TNFcausesapoptoticcelldeath,cellularproliferation,differentiation,inflammation,tumOrigenesisandviralreplication,TNFisalsoinvolvedinlipidmetabolism,andcoagulation.TNF"sprimaryroleisintheregulationofimmunecells.Dysregulationand,inparticular,overproductionofTNFhavebeenimplicatedinavarietyofhumandiseases-autoimmunediseases,insulinresistance,andcancer.
Description
TNFARatRecombinantproducedinE.Coliisasingle,non-glycosylatedpolypeptidechaincontaining181aminoacids(80-235a.a)andhavingamolecularmassof19.9kDa.TNFARatisexpressedwithan25aminoacidHistagatC-Terminusandpurifiedbyproprietarychromatographictechniques.
Source
PhysicalAppearance
Formulation
TNFARatproteinsolution(1mg/ml)containsPhosphateBufferedSaline(pH7.4),10%glyceroland1mMDTT.
StABIlity
Purity
Greaterthan90.0%asdeterminedbySDS-PAGE.
BIOLOGicalActivity
MeasuredinacytotoxicityassayusingL929mousefibrosarcomacellsinthepresenceofthemetabolicinhibitoractinomycinD.TheED50forthiseffectis≤to0.2ng/ml.
Aminoacidsequence
MGSSHHHHHHSSGLVPRGSHMGSHMLRSSSQNSSDKPVAHVVANHQAEEQLEWLSQRANALLANGMDLKDNQLVVPADGLYLIYSQVLFKGQGCPDYVLLTHTVSRFAISYQEKVSLLSAIKSPCPKDTPEGAELKPWYEPMYLGGVFQLEKGDLLSAEVNLPKYLDITESGQVYFGVIAL.
SafetyDataSheet
SDS
Usage
Prospec"sproductsarefurnishedforLABORATORYRESEARCHUSEONLY.Theproductmaynotbeusedasdrugs,agriculturalorpesticidalproducts,foodadditivesorhouseholdchemicals.
