
Cataloguenumber
CYT-1104
Synonyms
TNF-alpha,Tumornecrosisfactorligandsuperfamilymember2,TNF-a,Cachectin,DIF,TNFA,TNFSF2.
Introduction
Tumornecrosisfactorisamemberofagroupofcytokinesthatallstimulatetheacutephasereaction.TNFisinvolvedinsystemicinflammationandsecretedmainlybymacrophages.TNFcausesapoptoticcelldeath,cellularproliferation,differentiation,inflammation,tumOrigenesisandviralreplication,TNFisalsoinvolvedinlipidmetabolism,andcoagulation.TNF"sprimaryroleisintheregulationofimmunecells.Dysregulationand,inparticular,overproductionofTNFhavebeenimplicatedinavarietyofhumandiseases-autoimmunediseases,insulinresistance,andcancer.
Description
TNFABovineproducedinE.Coliisasingle,non-glycosylatedpolypeptidechaincontaining158aminoacids(78-234a.a.)andhavingamolecularmassof17.5kDa.
TNFAispurifiedbyproprietarychromatographictechniques.
Source
EscherichiaColi.
PhysicalAppearance
Sterilefilteredcolorlesssolution.
Formulation
TheTNFA(1mg/ml)containsPhosphatebuffersaline(pH7.4)and10%glycerol.
StABIlity
Storeat4°Cifentirevialwillbeusedwithin2-4weeks.Store,frozenat-20°Cforlongerperiodsoftime.Forlongtermstorageitisrecommendedtoaddacarrierprotein(0.1%HSAorBSA).Avoidmultiplefreeze-thawcycles.
Purity
Greaterthan90.0%asdeterminedbySDS-PAGE.
BIOLOGicalActivity
TheED50Is<15ng/mlandismeasuredinacytotoxicityassayusingL929mousefibrosarcomacellsinthepresenceofthemetabolicinhibitoractinomycinD.
Aminoacidsequence
MLRSSSQASSNKPVAHVVADINSPGQLRWWDSYANALMANGVKLEDNQLVVPADGLYLIYSQVLFRGQGCPSTPLFLTHTISRIAVSYQTKVNILSAIKSPCHRETPEWAEAKPWYEPIYQGGVFQLEKGDRLSAEINLPDYLDYAESGQVYFGIIAL.
SafetyDataSheet
SDS