
Cataloguenumber
CYT-1135
Synonyms
ThymicStromalLymphopoietin,TSLP.
Introduction
TSLPproteinisahemopoieticcytokinewhichsignalsthroughoutaheterodimericreceptorcomplexcomposedofthethymicstromallymphopoietinreceptor&theInterleukin-7receptoralphachain.TSLPimpactsmyeloidcellsthusinducesthedischargeofTcell-attractingchemokinesfrommonocytes&increasesthegrowthofCD11c(+)dendriticcells.TSLPismainlyexpressedintheheart,liverandprostate.TSLPisrelatedinitsBIOLOGicalactivitieswithIL-7andbindswiththeheterodimericreceptorcomplexconsistingoftheInterleukin-7receptoralphachain&theTSLPR.SimilartoIL-7,TSLPenhancesphosphorylationofSTAT3andSTAT5,thoughuseskinasesexcludingJAKsforitsactivation.TSLPinducesthereleaseofTcell-attractingchemokinessuchasTARC&MDCfrommonocytes&triggersCD11c(+)dendriticcells.TSLPactivateddendriticcellsprimesnaiveTcellstomanufacturepro-allergiccytokinessuchasIinterleukin-4,Interleukin-5,Interleukin-13andTNF-alphawhereasdown-regulatingInterleukin-10andIFN-gammaplayaroleintheinitiationofallergicinflammation.
Description
TSLPMouseRecombinantproducedinBaculovirusisasingleglycosylatedpolypeptidechaincontaining130aminoacids(20-140aa)andhavingamolecularmassof15.0kDa.TSLPisfusedtoa29aminoacidHis-TagatC-terminusandpurifiedbyproprietarychromatographictechniques.
Source
Sf9,Baculoviruscells.
PhysicalAppearance
Formulation
TheTSLPsolution(0.5mg/ml)containsPhosphateBufferedSaline(pH7.4)and10%glycerol.
StABIlity
Purity
Greaterthan90.0%asdeterminedbySDS-PAGE.
Aminoacidsequence
ADPYNFSNCNFTSITKIYCNIIFHDLTGDLKGAKFEQIEDCESKPACLLKIEYYTLNPIPGCPSLPDKTFARRTREALNDHCPGYPETERNDGTQEMAQEVQNICLNQTSQILRLWYSFMQSPEHHHHHH
SafetyDataSheet
SDS