Cataloguenumber
CYT-457
Synonyms
Cysteine-richsecretedproteinFIZZ3,
ADIposetissue-specificsecretoryfactor,ADSF,C/EBP-epsilon-regulatedmyeloid-specificsecretedcysteine-richprotein,Cysteine-richsecretedproteinA12-alpha-like2,RSTN,XCP1,RETN1,MGC126603,MGC126609.
Introduction
Resistin,aproductoftheRSTNgene,isapeptidehormonebelongingtotheclassofcysteine-richsecretedproteinswhichistermedtheRELMfamily,andisalsodescribedasADSF(AdiposeTissue-SpecificSecretoryFactor)andFIZZ3(FoundinInflammatoryZone).Humanresistincontains108aminoacidsasaprepeptide,anditshydrofobicsignalpeptideiscleavedbeforeitssecretion.Resistincirculatesinhumanbloodasadimericproteinconsistingoftwo92aminoacidpolypeptides,whicharedisulfide-linkedviaCys26.Resistinmaybeanimportantlinkbetweenobesityresistance.Mouseresistin,specificallyproducedandsecretedbyadipocyte,actsonskeletalmusclemyocytes,hepatocytesandadipocytesthemselvessothatitreducestheirsensitivity.Theyhavealsosuggestedthatresistinispresentatelevatedlevelsinbloodofobesemice,andisdownregulatedbyfastingandantidiabeticdrugs.Wayetal.,ontheotherhand,havefoundthatresistinexpressionisseverlysuppressedinobesityandisstimulatedbyseveralantidiabeticdrugs.Otherstudieshaveshownthatmouseresistinincreasesduringthedifferentiationofadipocytes,butitalsoseemstoinhibitAdipogenesis.Incontrast,thehumanadipogenicdifferentiationislikelytobeassociatedwithadownregulationofresistingeneexpression.
Description
ResistinMouseismanufacturedwithsignalsequenceofphagefd(21aa)andC-terminalfusionofflagTag(10aa).ResistinMouseRecombinantFlag-TaggedFusionProteinis13.7kDaproteincontaining93aminoacidresiduesoftheResistinMouseand31additionalaminoacidresidues-signalsequenceofphagefd,flagTag(underlined).
Source
EscherichiaColi.
PhysicalAppearance
FilteredWhitelyophilized(freeze-dried)powder.
Formulation
Filtered(0.4µm)andlyophilizedfrom0.5mg/mlin0.05MAcetatebufferpH-4.
Solubility
Itisrecommendedtoadd0.1MAcetatebufferpH4toprepareaworkingstocksolutionofapproximately0.5mg/mLandletthelyophilizedpelletdissolvecompletely.ForconversionintohigherpHvalue,werecommendintensivedilutionbyrelevantbuffertoaconcentrationof10μg/ml.Inhigherconcentrationsthesolubilityofthisantigenislimited.Productisnotsterile!Pleasefiltertheproductbyanappropriatesterilefilterbeforeusingitinthecellculture.
Storelyophilizedproteinat-20°C.Aliquottheproductafterreconstitutiontoavoidrepeatedfreezing/thawingcycles.Reconstitutedproteincanbestoredat4°Cforalimitedperiodoftime;itdoesnotshowanychangeaftertwoweeksat4°C.
Aminoacidsequence
MKKLLFAIPLVVPFYSHSTMASMPLCPIDEAIDKKIKQDFNSLFPNAIKNIGLNCWTVSSRGKLASCPEGTAVLSCSCGSACGSWDIREEKVCHCQCARIDWTAARCCKLQVASLEDYKDDDDK.
Purity
Greaterthan95%asdeterminedbySDS-PAGE.
SafetyDataSheet
Usage
Prospec"sproductsarefurnishedforLABORATORYRESEARCHUSEONLY.Theproductmaynotbeusedasdrugs,agriculturalorpesticidalproducts,foodadditivesorhouseholdchemicals.