
Cataloguenumber
CYT-1035
Synonyms
Resistin-likegamma,RELMgamma,RELM-γ,RELM-g.
Introduction
RELM-gammaisanovelmemberoftheresistin-likemolecule/foundininflammatoryzone(RELM/FIZZ)familyinmiceandrats.Microarrayandreal-timeRT-PCRexperimentsrevealedarepressionofRELMgammamRNAinnasalrespiratoryepitheliumofcigarettesmoke-exposedversusuntreatedrats.Theanalysisofthephysiologicaltissue-specificexpressionrevealedhighestexpressioninhematopoietictissues,suggestingacytokine-likeroleforRELM-gamma.RELM-gamma-mRNAisdetectableinbonemarrow,spleen,andlungaswellasinperipheralbloodgranulocytes.PromyelocyticHL60cellstransfectedwithaRELM-gammaexpressionplasmidhaveanincreasedproliferationratecomparedtomock-transfectedcellsanddisplayanalteredresponsetoretinoicacid-inducedgranulocyticdifferentiation.Takentogether,thesedataprovidethefirstexperimentalevidencethatRELM-gammaisasecretedmoleculewitharestrictedexpressionpatternthatmayplayaroleinpromyelocyticdifferentiation.
Description
RELMgMouseRecombinantproducedinE.Coliisanonglycosylated,homodimericpolypeptidechaincontaining2x89aminoacidsandhavingatotalmolecularmassof18.9kDa.
TheRELMispurifiedbyproprietarychromatographictechniques.
Source
EscherichiaColi.
PhysicalAppearance
Formulation
Lyophilizedfromasterilefilteredsolutioncontaining0.1%trifluoroaceticacid(TFA).
Solubility
ItisrecommendedtoreconstitutethelyophilizedRELMginsterile18MΩ-cmH2Oataconcentrationof100µg/ml,whichcanthenbefurtherdilutedtootheraqueoussolutions.
StABIlity
LyophilizedRELMgalthoughstableatroomtemperaturefor3weeks,shouldbestoreddesiccatedbelow-18°C.UponreconstitutionRELMgMouseshouldbestoredat4°Cbetween2-7daysandforfutureusebelow-18°C.
Forlongtermstorageitisrecommendedtoaddacarrierprotein(0.1%HSAorBSA).
Pleasepreventfreeze-thawcycles.
Purity
Aminoacidsequence
MEGTLESIVEKKVKELLANRDDCPSTVTKTFSCTSITASGRLASCPSGMTVTGCACGYGCGSWDIRDGNTCHCQCSTMDWATARCCQLA.
SafetyDataSheet
SDS