
Cataloguenumber
CYT-1097
Synonyms
BCGF,BCDF,Bcellstimulatingfactor,BSF-1,Lymphocytestimulatoryfactor1,IL-4,MGC79402,Binetrakin,Pitrakinra.
Introduction
IL4isapleiotropiccytokineproducedbyactivatedTcells.IL4isaligandforinterleukin4receptor.Theinterleukin4receptoralsobindstoIL13,whichcontributestovariousoverlappingrolesofthiscytokineandIL13.STAT6,asignaltransducerandactivatoroftranscriptionplaysamainroleinmediatingtheimmuneregulatorysignalofthiscytokine.IL4,IL3,IL5,IL13,andCSF2formacytokinegeneclusteronchromosome5q,withthisgeneparticularlyclosetoIL13.IL4,IL13andIL5areregulatedco-ordinatelybyseverallong-rangeregulatoryelementsinanover120kilobaserangeonthechromosome.
Description
Interleukin-4CanineRecombinantproducedinE.coliisanon-glycosylatedmonomerchaincontaining109aminoacidsandhavingamolecularmassof12.9kDa.
IL-4ispurifiedbyproprietarychromatographictechniques.
Source
EscherichiaColi.
PhysicalAppearance
Formulation
Theproteinwaslyophilizedfromasterile(0.2µm)filteredsolutioncontaining0.1%TrifluoroaceticAcid(TFA).
Solubility
ItisrecommendedtoreconstitutethelyophilizedIL-4insterile18MΩ-cmH2Onotlessthan100µg/ml,whichcanthenbefurtherdilutedtootheraqueoussolutions.
StABIlity
LyophilizedIL-4althoughstableatroomtemperaturefor3weeks,shouldbestoreddesiccatedbelow-18°C.UponreconstitutionIL-4shouldbestoredat4°Cbetween2-7daysandforfutureusebelow-18°C.
Pleasepreventfreeze-thawcycles.
Aminoacidsequence
MHNFNITIKEIIKMLNILTARNDSCMELTVKDVFTAPKNTSDKEIFCRAATVLRQIYTHNCSNRYLRGLYRNLSSMANKTCSMNEIKKSTLKDFLERLKVIMQKKYYRH.
Purity
Greaterthan95.0%asdeterminedbySDS-PAGE.
BIOLOGicalActivity
TheED50,asdeterminedbyTF-1cellproliferationis<25ng/mlcorrespondingtoaspecificactivitywhichis≥4.0x10^4units/mg.
SafetyDataSheet
SDS