Cataloguenumber
CYT-101
Synonyms
NKSF,CTLmaturationfactor(TCMF),Cytotoxiclymphocytematurationfactor(CLMF),TSF,Edodekin-alpha,IL-12.
Introduction
IL-12isaheterodimericcytokinethatstimulatestheproductionofIFNgammafromT-cellsandnaturalkillercells,andalsoinducesdifferentiationofTh1helpercells.IL-12isaninitiatorofcell-mediatedimmunity.
Description
Interleukin-12HumanRecombinantproducedinHEKcellsisaglycosylatedheterodimer,havingatotalmolecularweightof57kDa.TheIL12ispurifiedbyproprietarychromatographictechniques.
Source
HEK.
PhysicalAppearance
SterileFilteredWhitelyophilized(freeze-dried)powder.
Formulation
IL12waslyophilizedfroma0.2µmfilteredsolutioncontaining1xPBS.
Solubility
ItisrecommendedtoreconstitutethelyophilizedIL12insterilePBScontaining0.1%endotoxin-freerecombinantHSA.
LyophilizedIL-12althoughstableatroomtemperaturefor3weeks,shouldbestoreddesiccatedbelow-18°C.UponreconstitutionIL12shouldbestoredat4°Cbetween2-7daysandforfutureusebelow-18°C.Forlongtermstorageitisrecommendedtoaddacarrierprotein(0.1%HSAorBSA).Pleasepreventfreeze-thawcycles.
Purity
Greaterthan95%asobserevedbySDS-PAGE.
Aminoacidsequence
IL-12isaheterodimerofIL-12AandIL-12Blinkedthroughadisulfide-bondbetweencysteinesinredinsequencesbelow.>IL-12ARNLPVATPDPGMFPCLHHSQNLLRAVSNMLQKARQTLEFYPCTSEEIDHEDITKDKTSTVEACLPLELTKNESCLNSRETSFITNGSCLASRKTSFMMALCLSSIYEDLKMYQVEFKTMNAKLLMDPKRQIFLDQNMLAVIDELMQALNFNSETVPQKSSLEEPDFYKTKIKLCILLHAFRIRAVTIDRVMSYLNAS.>IL-12BIWELKKDVYVVELDWYPDAPGEMVVLTCDTPEEDGITWTLDQSSEVLGSGKTLTIQVKEFGDAGQYTCHKGGEVLSHSLLLLHKKEDGIWSTDILKDQKEPKNKTFLRCEAKNYSGRFTCWWLTTISTDLTFSVKSSRGSSDPQGVTCGAATLSAERVRGDNKEYEYSVECQEDSACPAAEESLPIEVMVDAVHKLKYENYTSSFFIRDIIKPDPPKNLQLKPLKNSRQVEVSWEYPDTWSTPHSYFSLTFCVQVQGKSKREKKDRVFTDKTSATVICRKNASISVRAQDRYYSSSWSEWASVPCS.
SafetyDataSheet
Thespecificactivitywasdeterminedbythedose-dependentreleaseofIFN-gammafromthehumanNK92celllineinpresenceof20ng/mLrIL-2.TheEC50is0.5ng/ml.
Usage
ProspecsproductsarefurnishedforLABORATORYRESEARCHUSEONLY.Theproductmaynotbeusedasdrugs,agriculturalorpesticidalproducts,foodadditivesorhouseholdchemicals.