
Cataloguenumber
CYT-1098
Synonyms
BCGF,BCDF,Bcellstimulatingfactor,BSF-1,Lymphocytestimulatoryfactor1,IL-4,MGC79402,Binetrakin,Pitrakinra.
Introduction
IL4isapleiotropiccytokineproducedbyactivatedTcells.IL4isaligandforinterleukin4receptor.Theinterleukin4receptoralsobindstoIL13,whichcontributestovariousoverlappingrolesofthiscytokineandIL13.STAT6,asignaltransducerandactivatoroftranscriptionplaysamainroleinmediatingtheimmuneregulatorysignalofthiscytokine.IL4,IL3,IL5,IL13,andCSF2formacytokinegeneclusteronchromosome5q,withthisgeneparticularlyclosetoIL13.IL4,IL13andIL5areregulatedco-ordinatelybyseverallong-rangeregulatoryelementsinanover120kilobaserangeonthechromosome.
Description
Interleukin-4PorcineRecombinantproducedinE.coliisanon-glycosylatedmonomerchaincontaining110aminoacidsandhavingamolecularmassof12.7kDa.
IL-4ispurifiedbyproprietarychromatographictechniques.
Source
EscherichiaColi.
PhysicalAppearance
Formulation
Theproteinwaslyophilizedfromasterile(0.2µm)filteredsolutioncontaining10mMsodiumphosphate,pH7.5.
Solubility
ItisrecommendedtoreconstitutethelyophilizedIL-4insterile18MΩ-cmH2Onotlessthan100µg/ml,whichcanthenbefurtherdilutedtootheraqueoussolutions.
StABIlity
LyophilizedIL-4althoughstableatroomtemperaturefor3weeks,shouldbestoreddesiccatedbelow-18°C.UponreconstitutionIL-4shouldbestoredat4°Cbetween2-7daysandforfutureusebelow-18°C.
Pleasepreventfreeze-thawcycles.
Aminoacidsequence
MHKCDITLQEIIKTLNILTARKNSCMELPVTDVFAAPENTTEKETFCRASTVLRHIYRHHTCMKSLLSGLDRNLSSMANMTCSVHEAKKSTLKDFLERLKTIMKEKYSKC.
Purity
Greaterthan95.0%asdeterminedbySDS-PAGE.
BIOLOGicalActivity
TheED50,asdeterminedbyTF-1cellproliferationis0.504ng/mlcorrespondingtoaspecificactivitywhichis2.0x10^6units/mg.
SafetyDataSheet
SDS