
Cataloguenumber
CYT-1018
Synonyms
Interleukin-2,IL-2,T-cellgrowthfactor,TCGF,IL2.
Introduction
IL2isasecretedcytokinethatisimportantfortheproliferationofTandBlymphocytes.Thereceptorofthiscytokineisaheterotrimericproteincomplexwhosegammachainisalsosharedbyinterleukin4(IL4)andinterleukin7(IL7).Theexpressionofthisgeneinmaturethymocytesismonoallelic,whichrepresentsanunusualregulatorymodeforcontrollingthepreciseexpressionofasinglegene.Thetargeteddisruptionofasimilargeneinmiceleadstoulcerativecolitis-likedisease,whichsuggestsanessentialroleofthisgeneintheimmuneresponsetoantigenicstimuli.
Description
IL2FelineRecombinantproducedinE.Coliisasingle,non-glycosylatedpolypeptidechaincontaining157aminoacids(21-154a.a)andhavingamolecularmassof17.8kDa.IL2isfusedtoa23aminoacidHis-tagatN-terminus&purifiedbyproprietarychromatographictechniques.
Source
PhysicalAppearance
Formulation
IL2proteinsolution(0.25mg/ml)containing20mMTris-HCl(pH8.0),0.15MNaCland30%glycerol.
StABIlity
Purity
Greaterthan95%asdeterminedbySDS-PAGE.
BIOLOGicalActivity
MeasuredinacellproliferationassayusingCTLL2mousecytotoxicTcells.TheED50forthiseffectislessorequalto0.3ng/ml.
Aminoacidsequence
MGSSHHHHHHSSGLVPRGSHMGSAPASSSTKETQQQLEQLLLDLRLLLNGVNNPENPKLSRMLTFKFYVPKKATELTHLQCLVEELKPLEEVLYLAQSKNFHLNHIKELMSNINVTVLKLKGSETRFTCNYDDETATIVEFLNKWITFCQSIFSTLT.
SafetyDataSheet
SDS