Cataloguenumber
CYT-1011
Synonyms
Interleukin-2,IL-2,T-cellgrowthfactor,TCGF.
Introduction
IL2isasecretedcytokinethatisimportantfortheproliferationofTandBlymphocytes.Thereceptorofthiscytokineisaheterotrimericproteincomplexwhosegammachainisalsosharedbyinterleukin4(IL4)andinterleukin7(IL7).Theexpressionofthisgeneinmaturethymocytesismonoallelic,whichrepresentsanunusualregulatorymodeforcontrollingthepreciseexpressionofasinglegene.Thetargeteddisruptionofasimilargeneinmiceleadstoulcerativecolitis-likedisease,whichsuggestsanessentialroleofthisgeneintheimmuneresponsetoantigenicstimuli.
Description
IL2CanineRecombinantproducedinE.Coliisasingle,non-glycosylatedpolypeptidechaincontaining160aminoacids(21-155a.a)andhavingamolecularmassof18.1kDa.IL2Canineisfusedtoa25aminoacidHis-tagatN-terminus&purifiedbyproprietarychromatographictechniques.
Source
PhysicalAppearance
Sterilefilteredcolorlesssolution.
Formulation
IL2Canineproteinsolution(0.5mg/ml)containsPhosphatebufferedsaline(pH7.4).
StABIlity
Purity
Greaterthan95.0%asdeterminedbySDS-PAGE.
Aminoacidsequence
MGSSHHHHHHSSGLVPRGSHMGSHMAPITSSSTKETEQQMEQLLLDLQLLLNGVNNYENPQLSRMLTFKFYTPKKATEFTHLQCLAEELKNLEEVLGLPQSKNVHLTDTKELISNMNVTLLKLKGSETSYNCEYDDETATITEFLNKWITFSQSIFSTLT.
BIOLOGicalActivity
TheED50measuredinacellproliferationassayusingCTLL-2mousecytotoxicTcells.is<0.3ng/ml.
SafetyDataSheet
SDS

