
Cataloguenumber
CYT-1056
Synonyms
IL1R2,CD121b,CDw121b,IL-1R-2,IL-1RT-2,IL-1RT2,IL1R2c,IL1RB,IL-1typeIIreceptor,Interleukin-1receptorbeta,CD121antigen-likefamilymemberB.
Introduction
IL1R2isacytokinereceptorwhichisapartoftheinterleukin-1receptorfamily.IL1R2isexpressedmainlybyTcells,endothelialcellsandfibroblasts.IL1R2bindsIL1A,IL1BandIL1RNpreventingthemfrombindingtotheirregularreceptorsandthusinhibitingthetransductionoftheirsignaling.Also,Interleukin-4antagonizestheactivityofinterleukin-1byinducingtheexpressionandreleaseofthisprotein.
Description
IL1R2producedinSf9Insectcellsisasingle,glycosylatedpolypeptidechaincontaining338aminoacids(14-343a.a.)andhavingamolecularmassof38.8kDa(MolecularsizeonSDS-PAGEwillappearatapproximately40-57kDa).IL1R2isexpressedwithan8aminoacidHistagatC-Terminusandpurifiedbyproprietarychromatographictechniques.
Source
Sf9,Insectcells.
PhysicalAppearance
Formulation
IL1R2proteinsolution(0.5mg/ml)containsPBS(pH7.4)and10%glycerol.
StABIlity
Purity
Greaterthan90%asdeterminedbySDS-PAGE.
BIOLOGicalActivity
MeasuredbyitsabilitytoinhibitproliferationusingD10.G4.1mousehelperTcells.TheED50forthiseffectis≤to400ng/mlwithIL-1beta.
Aminoacidsequence
FTLQPAAHTGAARSCRFRGRHYKREFRLEGEPVALRCPQVPYWLWASVSPRINLTWHKNDSARTVPGEEETRMWAQDGALWLLPALQEDSGTYVCTTRNASYCDKMSIELRVFENTDAFLPFISYPQILTLSTSGVLVCPDLSEFTRDKTDVKIQWYKDSLLLDKDNEKFLSVRGTTHLLVHDVALEDAGYYRCVLTFAHEGQQYNITRSIELRIKKKKEETIPVIISPLKTISASLGSRLTIPCKVFLGTGTPLTTMLWWTANDTHIESAYPGGRVTEGPRQEYSENNENYIEVPLIFDPVTREDLHMDFKCVVHNTLSFQTLRTTVKELEHHHHHH.
SafetyDataSheet
SDS