Cataloguenumber
CYT-1134
Synonyms
Interleukin-1alpha,IL-1alpha,Hematopoietin-1,Interleukin-1alpha:Interleukin-1a,IL-1a,Interleukin-1alpha,IL1A,interleukin1alpha,IL1F1,IL-1A,preinterleukin1alpha,pro-interleukin-1-alpha.
Introduction
IL-1alphaisderivedfromactivatedmacrophages.IL1Aproteinstakepartintheinflammatoryresponse,beingidentifiedasendogenouspyrogens,andstimulatethereleaseofprostaglandinandCollagenasefromsynovialcells.IL-1alphastimulatesthymocyteproliferationbyinducingil-2release,b-cellmaturationandproliferation,andfibroblastgrowthfactoractivity.
Description
IL1AHumanRecombinantproducedinE.Coliisasingle,non-glycosylatedpolypeptidechaincontaining197aminoacids(113-271a.a)andhavingamolecularmassof22.4kDa.IL1Aisfusedtoa38aminoacidHis-tagatN-terminus&purifiedbyproprietarychromatographictechniques.
Source
PhysicalAppearance
Formulation
IL1Aproteinsolution(1mg/ml)contains20mMTris-HCl(pH7.5)and10%glycerol.
StABIlity
Purity
Greaterthan90%asdeterminedbySDS-PAGE.
BIOLOGicalActivity
MeasuredinacellproliferationassayusingD10.G4.1mousehelperTcell.TheED50forthiseffectislessorequalto0.04ng/ml.
Aminoacidsequence
MRGSHHHHHHGMASMTGGQQMGRDLYDDDDKDRWGSHMSAPFSFLSNVKYNFMRIIKYEFILNDALNQSIIRANDQYLTAAALHNLDEAVKFDMGAYKSSKDDAKITVILRISKTQLYVTAQDEDQPVLLKEMPEIPKTITGSETNLLFFWETHGTKNYFTSVAHPNLFIATKQDYWVCLAGGPPSITDFQILENQA.
SafetyDataSheet
SDS

