Cataloguenumber
CYT-1050
Synonyms
Interleukin-18receptoraccessoryprotein,IL-18receptoraccessoryprotein,IL-18RAcP,Accessoryprotein-like,AcPL,CD218antigen-likefamilymemberB,CDw218b,IL-1Raccessoryprotein-like,IL-1RAcPL,Interleukin-1receptor7,IL-1R-7,IL-1R7,Interleukin-18receptoraccessoryprotein-like,Interleukin-18receptorbeta,IL-18R-beta,IL-18Rbeta,CD218b,IL1R7.
Introduction
Interleukin-18receptoraccessoryprotein(IL18RAP),belongstotheIL-1familyofcytokineswhichholdsmultipleimmunoregulatoryfunctions.IthasbeenfoundthatIL18RAPdoesnotmediateIL18-binding,however,IL18RAPisinvolvedinIL18-dependentsignaltransduction,whichleadstoNF-kappa-BandJNKactivation.IL18R1aswellasIL18RAPpolymorphismshavebeenlinkedwiththefollowingdiseases:schizophrenia,HSV1SEROpositivityandatopicasthma.
Description
IL18RAPproducedinSf9Insectcellsisasingle,glycosylatedpolypeptidechaincontaining576aminoacids(20-356a.a.)andhavingamolecularmassof65.4kDa(MolecularsizeonSDS-PAGEwillappearatapproximately70-100kDa).IL18RAPisexpressedwithan239aminoacidHistagatC-Terminusandpurifiedbyproprietarychromatographictechniques.
Source
Sf9,Insectcells.
PhysicalAppearance
Sterilefilteredcolorlesssolution.
Formulation
IL18RAPproteinsolution(0.25mg/ml)containsPhosphateBufferedSaline(pH7.4)AND10%glycerol.
StABIlity
Storeat4°Cifentirevialwillbeusedwithin2-4weeks.Store,frozenat-20°Cforlongerperiodsoftime.Forlongtermstorageitisrecommendedtoaddacarrierprotein(0.1%HSAorBSA).Avoidmultiplefreeze-thawcycles.
Purity
Greaterthan90.0%asdeterminedbySDS-PAGE.
Aminoacidsequence
FNISGCSTKKLLWTYSTRSEEEFVLFCDLPEPQKSHFCHRNRLSPKQVPEHLPFMGSNDLSDVQWYQQPSNGDPLEDIRKSYPHIIQDKCTLHFLTPGVNNSGSYICRPKMIKSPYDVACCVKMILEVKPQTNASCEYSASHKQDLLLGSTGSISCPSLSCQSDAQSPAVTWYKNGKLLSVERSNRIVVDEVYDYHQGTYVCDYTQSDTVSSWTVRAVVQVRTIVGDTKLKPDILDPVEDTLEVELGKPLTISCKARFGFERVFNPVIKWYIKDSDLEWEVSVPEAKSIKSTLKDEIIERNIILEKVTQRDLRRKFVCFVQNSIGNTTQSVQLKEKRLEPKSCDKTHTCPPCPAPELLGGPSVFLFPPKPKDTLMISRTPEVTCVVVDVSHEDPEVKFNWYVDGVEVHNAKTKPREEQYNSTYRVVSVLTVLHQDWLNGKEYKCKVSNKALPAPIEKTISKAKGQPREPQVYTLPPSRDELTKNQVSLTCLVKGFYPSDIAVEWESNGQPENNYKTTPPVLDSDGSFFLYSKLTVDKSRWQQGNVFSCSVMHEALHNHYTQKSLSLSPGKHHHHHH.
SafetyDataSheet
SDS
