Cataloguenumber
CYT-1017
Synonyms
CytokineML-1,IL-17F,Interleukin-17Fprecursor,IL17F,ML1,ML-1.
Introduction
IL-17FhavinganaccessionnumberofQ96PD4isacytokinethatsharessequencesimilaritywithIL17.IL-17FisexpressedbyactivatedTcells,andhasbeenshowntostimulatetheproductionofseveralothercytokines,includingIL6,IL8,andCSF2/GM_CSF.IL-17FinhibitstheangiogenesisofendothelialcellsandinduceendothelialcellstoproduceIL2,TGFB1/TGFB,andmonocytechemoattractantprotein-1.IL-17Finducesstromalcellstoproduceproinflammatoryandhematopoieticcytokines.IntestinalIL17FgeneexpressionisincreasedinactiveCD.IL-17A&IL-17Fallelesinfluencethesusceptibilitytoandpathophysiologicalfeaturesofulcerativecolitisindependently.IL-17FandMIFgenepolymorphismsaresignificantlyassociatedwiththedevelopmentoffunctionaldyspepsia.TheinitiationofIL-17F/IL-17RsignalingpathwayrequiresthereceptorubiquitinationbyTRAF6.IL-17FinducesexpressionofIFN-gamma-inducIBLeprotein10(IP-10)byactivatingRaf1-mitogen-activatedproteinkinase1/2-extracellular-regulatedkinase1/2-p90ribosomalS6kinase-cyclicAMPresponseelement-bindingproteinsignalingpathway.
Description
IL17FproducedinSf9Baculoviruscellsisasingle,glycosylatedpolypeptidechaincontaining142aminoacids(31-163a.a.)andhavingamolecularmassof16kDa(MolecularsizeonSDS-PAGEwillappearatapproximately18-28kDa).IL17Fisexpressedwitha9aminoacidHistagatC-Terminusandpurifiedbyproprietarychromatographictechniques.
Source
PhysicalAppearance
Formulation
IL17Fproteinsolution(0.5mg/ml)containsPhosphateBufferedSaline(pH7.4),20%glyceroland1mMDTT.
StABIlity
Purity
Greaterthan95.0%asdeterminedbySDS-PAGE.
Aminoacidsequence
ADPRKIPKVGHTFFQKPESCPPVPGGSMKLDIGIINENQRVSMSRNIESRSTSPWNYTVTWDPNRYPSEVVQAQCRNLGCINAQGKEDISMNSVPIQQETLVVRRKHQGCSVSFQLEKVLVTVGCTCVTPVIHHVQHHHHHH.
SafetyDataSheet
SDS

