
Cataloguenumber
CYT-1037
Synonyms
Interleukin15ReceptorSubunitAlpha,Interleukin15Receptor,Alpha,Interleukin15ReceptorAlphaIsoformEM2,Interleukin15ReceptorAlphaIsoformIC2,Interleukin15ReceptorAlphaIsoformIC3,Interleukin15ReceptorAlphaIsoformIC4,Interleukin15ReceptorAlphaIsoformIC5,Interleukin15ReceptorAlphaIsoformIC6,Interleukin15ReceptorAlphaIsoformIC7,Interleukin15ReceptorAlphaIsoformIC8,Interleukin-15ReceptorSubunitAlpha,IL-15ReceptorSubunitAlpha,CD215Antigen,IL-15R-Alpha,IL-15RA,CD215.
Introduction
IL15RA,alsoknownasInterleukin15ReceptorAlpha,isahigh-affinityreceptorforinterleukin-15.IL15RAplaysaroleasaheterotrimerwiththeIL-2receptorbetaandgammasubunitstocommencesignaltransduction.IL15/IL15Ralphacomplexescantransmitreversesignalingwhichpromotescellularadhesion,tyrosinephosphorylationofintracellularproteins,aswellascytokinesecretionbytheIL‑15/IL15Ralphaexpressingcells.
Description
IL15RAproducedinSf9Baculoviruscellsisasingle,glycosylatedpolypeptidechain(31-205a.a.)andfusedtoa242a.a.hIgG-HisTagatC-terminuscontainingatotalof417aminoacidsandhavingamolecularmassof45.6kDa.IL15RAshowsmultiplebandsbetween57-70kDaonSDS-PAGE,reducingconditionsandpurifiedbyproprietarychromatographictechniques.
Source
PhysicalAppearance
Formulation
IL15RAproteinsolution(0.5mg/ml)containsPhosphatebufferedsaline(pH7.4)and10%glycerol.
StABIlity
Purity
Greaterthan90.0%asdeterminedbySDS-PAGE.
BIOLOGicalActivity
DeterminedbyitsabilitytoinhibitproliferationusingCTLL2mousecytotoxicTcells.TheED50forthiseffectis<2ng/mlwithHumanIL-15.
Aminoacidsequence
ADPITCPPPMSVEHADIWVKSYSLYSRERYICNSGFKRKAGTSSLTECVLNKATNVAHWTTPSLKCIRDPALVHQRPAPPSTVTTAGVTPQPESLSPSGKEPAASSPSSNNTAATTAAIVPGSQLMPSKSPSTGTTEISSHESSHGTPSQTTAKNWELTASASHQPPGVYPQGHSDTTLEPKSCDKTHTCPPCPAPELLGGPSVFLFPPKPKDTLMISRTPEVTCVVVDVSHEDPEVKFNWYVDGVEVHNAKTKPREEQYNSTYRVVSVLTVLHQDWLNGKEYKCKVSNKALPAPIEKTISKAKGQPREPQVYTLPPSRDELTKNQVSLTCLVKGFYPSDIAVEWESNGQPENNYKTTPPVLDSDGSFFLYSKLTVDKSRWQQGNVFSCSVMHEALHNHYTQKSLSLSPGKHHHHHH
SafetyDataSheet
SDS