
Cataloguenumber
CYT-1058
Synonyms
Interleukin12(subunitbeta/alpha),IL12b/IL12a,Il-12b/Il-12a,IL-12p40/Il-12p35,Il12p40/Il12p35,p40/p35,Sf9.
Introduction
interleukin12subunitbeta/alphaorIL12b/IL12aisagrowthfactorcytokinewhichincreasesthelyticactivityofNK/lymphokine-activatedkillercells,activatedTandNKcellsandpromptIFN-gammaproduction(throughrestingPBMC).IL12b/IL12aisacrucialparttotheprocessofcellular-immunityandactivatesthedifferentiationofTh1cellsoriginatesfromtheprecursorThelpercells.TheproteinislinkedtoIL23AandcreatestheIL-23interleukin,bythat,theautoimmuneinflammationisinducedandautoimmuneinflammatorydiseasesandtumOrigenesisarebeingaffected.
Description
IL12MouseRecombinantproducedinabaculovirusexpressionsystemisaglycosylateddisulfidelinked(throughcysteinesinbold)heterodimercomprisedofIL12A(23-335aa,totalof319aa,MW35.7kDa)andIL12B(23-215aa,totalof199aa,MW22.5kDa),havingatotalpredictedmolecularmassof58.3kDa(MolecularweightonSDS-PAGEwillappearhigher).IL12Aisfusedtoa6aminoacidHis-TagatC-terminusandpurifiedbyproprietarychromatographictechniques.
Source
Sf9,Baculoviruscells.
PhysicalAppearance
Formulation
IL12proteinsolution(0.5mg/ml)containsphosphatebufferedsaline(pH7.4)and10%glycerol.
StABIlity
Purity
Greaterthan95.0%asdeterminedbySDS-PAGE.
Aminoacidsequence
IL12B(p40)MWELEKDVYVVEVDWTPDAPGETVNLTCDTPEEDDITWTSDQRHGVIGSGKTLTITVKEFLDAGQYTCHKGGETLSHSHLLLHKKENGIWSTEILKNFKNKTFLKCEAPNYSGRFTCSWLVQRNMDLKFNIKSSSSSPDSRAVTCGMASLSAEKVTLDQRDYEKYSVSCQEDVTCPTAEETLPIELALEARQQNKYENYSTSFFIRDIIKPDPPKNLQMKPLKNSQVEVSWEYPDSWSTPHSYFSLKFFVRIQRKKEKMKETEEGCNQKGAFLVEKTSTEVQCKGGNVCVQAQDRYYNSSCSKWACVPCRVRSIL12A(p35)RVIPVSGPARCLSQSRNLLKTTDDMVKTAREKLKHYSCTAEDIDHEDITRDQTSTLKTCLPLELHKNESCLATRETSSTTRGSCLPPQKTSLMMTLCLGSIYEDLKMYQTEFQAINAALQNHNHQQIILDKGMLVAIDELMQSLNHNGETLRQKPPVGEADPYRVKMKLCILLHAFSTRVVTINRVMGYLSSAHHHHHH.
SafetyDataSheet
SDS