Cataloguenumber
CYT-584
Synonyms
NK4,TAIF,TAIFa,TAIFb,TAIFc,TAIFd,IL-32beta,IL-32alpha,IL-32delta,IL-32gamma,Interleukin-32,IL-32,Naturalkillercellsprotein4,Tumornecrosisfactoralpha-inducingfactor,IL-32a,IL32a,IL32,Interleukin-32alpha.
Introduction
IL-32ispartofthecytokinefamilyandcontainsatyrosinesulfationsite,3potentialN-myristoylationsites,multipleputativephosphorylationsites,andanRGDcell-attachmentsequence.IL-32expressioniselevatedaftertheactivationofT-cellsbymitogensortheactivationofNKcellsbyIL-2.IL-32inducestheproductionofTNF-afrommacrophagecells.IL-32pro-inflammatorypathwayisactivatedinresponsetoinfluenzaAvirusinfection.DysregulationofIL-32inmyelodysplasticsyndromeandchronicmyelomonocyticleukemiamodulatesapoptosisandimpairsNKfunction.InductionofTNF,IL-1beta,andIL-6byIL-32isintervenedbyp38-MAPK.IL-32inducedmonocyte-to-macrophagedifferentiationismediatedthroughnonapoptotic,caspase-3-dependentmechanisms.IL32playsanimportantroleinthepathogenesisofrheumatoidarthritis.IL-32isinvolvedinactivation-inducedcelldeathinTcells,throughitsintracellularactions.IL-32isacell-associatedproinflammatorycytokine,whichisparticularlystimulatedbymycobacteriathroughacaspase-1-andIL-18-dependentproductionofIFNgamma.IL-32isassociatedwithTNF-a,IL-1beta,andIL-18.IL32isinvolvedinhumanrheumatoidarthritisandisanoveltargetinautoimmunediseases.
Description
Interleukin-32humanrecombinantproducedinE.Coliisasingle,non-glycosylated,polypeptidechaincontaining131aminoacidsandhavingamolecularmassof14.9kDa.
Source
EscherichiaColi.
PhysicalAppearance
SterileFilteredWhitelyophilized(freeze-dried)powder.
Formulation
IL-32waslyophilizedfromaconcentrated(1mg/ml)solutioninwatercontaining50mMsodiumPhosphatebufferpH=7.5.
Solubility
ItisrecommendedtoreconstitutethelyophilizedIL-32insterile18MΩ-cmH2Onotlessthan100µg/ml,whichcanthenbefurtherdilutedtootheraqueoussolutions.
LyophilizedIL32althoughstableatroomtemperaturefor3weeks,shouldbestoreddesiccatedbelow-18°C.UponreconstitutionIL32shouldbestoredat4°Cbetween2-7daysandforfutureusebelow-18°C.Pleasepreventfreeze-thawcycles.
Purity
Greaterthan97.0%asdeterminedby(a)AnalysisbyRP-HPLC.(b)AnalysisbySDS-PAGE.
HumanIL-32alphaactivityismeasuredviathedose-dependentinductionofTNF-alphainthehumanTHP-1monocyticcellline.
Aminoacidsequence
MCFPKVLSDDMKKLKARMHQAIERFYDKMQNAESGRGQVMSSLAELEDDFKEGYLETVAAYYEEQHPELTPLLEKERDGLRCRGNRSPVPDVEDPATEEPGESFCDKSYGAPRGDKEELTPQKCSEPQSSK.
SafetyDataSheet
Usage
Prospec"sproductsarefurnishedforLABORATORYRESEARCHUSEONLY.Theproductmaynotbeusedasdrugs,agriculturalorpesticidalproducts,foodadditivesorhouseholdchemicals.