
Cataloguenumber
CYT-520
Synonyms
IFN-alpha1a,IFN-a1a,IFNalpha1a.
Introduction
Description
IFN-alpha1aHumanRecombinantproducedinE.Coliisasingle,non-glycosylated,polypeptidechaincontaining167aminoacidsandhavingamolecularmassof19.5kDa.TheIFNA1A(V115A)ispurifiedbyproprietarychromatographictechniques.
Source
PhysicalAppearance
Formulation
TheIFN-a1aproteinwaslyophilizedfroma0.2µmfilteredconcentratedsolutioninPBS,pH7.4,containing3%Mannitol,5%Trehalose,0.05%Tween-80.
Solubility
StABIlity
LyophilizedIFNA1Aalthoughstableatroomtemperaturefor3weeks,shouldbestoreddesiccatedbelow-18°C.UponreconstitutionIFN-alpha1ashouldbestoredat4°Cbetween2-7daysandforfutureusebelow-18°C.Forlongtermstorageitisrecommendedtoaddacarrierprotein(0.1%HSAorBSA).Pleasepreventfreeze-thawcycles.
Purity
Greaterthan97.0%asdeterminedby:(a)AnalysisbyRP-HPLC.(b)AnalysisbySDS-PAGE.
BIOLOGicalActivity
Aminoacidsequence
MCDLPETHSLDNRRTLMLLAQMSRISPSSCLMDRHDFGFPQEEFDGNQFQKAPAISVLHELIQQIFNLFTTKDSSAAWDEDLLDKFCTELYQQLNDLEACVMQEERVGETPLMNADSILAVKKYFRRITLYLTEKKYSPCAWEVVRAEIMRSLSLSTNLQERLRRKE.
SafetyDataSheet
SDS