
Cataloguenumber
CYT-358
Synonyms
ImmuneIFN,typeIIIFN,TcellIFN,MAF,IFNG,IFG,IFI,IFN-gamma.
Introduction
IFN-gammaproducedbylymphocytesactivatedbyspecificantigensormitogens.IFN-gamma,inadditiontohavingantiviralactivity,hasimportantimmunoregulatoryfunctions,itisapotentactivatorofmacrophages,andhasantiproliferativeeffectsontransformedcellsanditcanpotentiatetheantiviralandantitumoreffectsofthetypeIIFNs.
Description
IFN-gammaMouseRecombinantproducedinE.Coliisasingle,non-glycosylated,polypeptidechaincontaining134aminoacidsandhavingamolecularmassof15.6kDa.TheIFN-gammaispurifiedbyproprietarychromatographictechniques.
Source
PhysicalAppearance
Formulation
Solubility
ItisrecommendedtoreconstitutethelyophilizedIFN-gammainsteriledistilledwateror20mMAcOHatconcentrationsrangingbetween0.1mg-0.5mg/ml,whichcanthenbefurtherdilutedtootheraqueoussolutions.
StABIlity
LyophilizedIFN-gammaalthoughstableatroomtemperaturefor3weeks,shouldbestoreddesiccatedbelow-18°C.UponreconstitutionIFN-gammashouldbestoredat4°Cbetween2-7daysandforfutureusebelow-18°C.Forlongtermstorageitisrecommendedtoaddacarrierprotein(0.1%HSAorBSA).Pleasepreventfreeze-thawcycles.
Purity
Aminoacidsequence
BIOLOGicalActivity
References
Title:IFN-γandinterleukin-4reciprocallyregulateCD8expressioninCD8+Tcells.Publication:PublishedonlinebeforeprintNovember6,2008,doi:10.1073/pnas.0809549105PNASNovember11,2008vol.105no.4517475-17480Link:http://www.pnas.org/content/105/45/17475.full
SafetyDataSheet
SDS