Cataloguenumber
CYT-206
Synonyms
ImmuneIFN,typeIIIFN,TcellIFN,MAF,IFNG,IFG,IFI,IFN-gamma.
Introduction
IFN-gammaproducedbylymphocytesactivatedbyspecificantigensormitogens.IFN-gamma,inadditiontohavingantiviralactivity,hasimportantimmunoregulatoryfunctions,itisapotentactivatorofmacrophages,andhasantiproliferativeeffectsontransformedcellsanditcanpotentiatetheantiviralandantitumoreffectsofthetypeIIFNs.
Description
IFN-gammaHumanRecombinantproducedinE.Coliisasingle,non-glycosylated,polypeptidechaincontaining144aminoacidsandhavingamolecularmassof17kDa.TheIFN-gammaispurifiedbyproprietarychromatographictechniques.
Source
EscherichiaColi.
PhysicalAppearance
SterileFilteredWhitelyophilized(freeze-dried)powder.
Formulation
Theproteinwaslyophilizedfroma0.2µmfilteredconcentratedsolutioninPBSpH4.6.
Solubility
ItisrecommendedtoreconstitutethelyophilizedIFN-gammainsteriledistilledwateror20mMAcOHnotlessthan100µg/ml,whichcanthenbefurtherdilutedtootheraqueoussolutions.
LyophilizedIFNgammaalthoughstableatroomtemperaturefor3weeks,shouldbestoreddesiccatedbelow-18°C.UponreconstitutionIFN-gammashouldbestoredat4°Cbetween2-7daysandforfutureusebelow-18°C.Forlongtermstorageitisrecommendedtoaddacarrierprotein(0.1%HSAorBSA).Pleasepreventfreeze-thawcycles.
Purity
Greaterthan98.0%asdeterminedby:(a)AnalysisbyRP-HPLC.(b)AnalysisbySDS-PAGE.
Aminoacidsequence
MQDPYVKEAENLKKYFNAGHSDVADNGTLFLGILKNWKEESDRKIMQSQIVSFYFKLFKNFKDDQSIQKSVETIKEDMNVKFFNSNKKKRDDFEKLTNYSVTDLNVQRKAIHELIQVMAELSPAAKTGKRKRSQMLFQGRRASQ.
Thespecificactivityasdeterminedinaviralresistanceassayis<0.05ng/ml,correspondingtoaspecificactivityof2.0x10,000,000IU/mg.
Proteincontent
Proteinquantitationwascarriedoutbytwoindependentmethods:1.UVspectroscopyat280nmusingtheabsorbencyvalueof0.640astheextinctioncoefficientfora0.1%(1mg/ml)solution.ThisvalueiscalculatedbythePCGENEcomputeranalysisprogramofproteinsequences(IntelliGenetics).2.AnalysisbyRP-HPLC,usingacalibratedsolutionofIFN-gasaReferenceStandard.
Usage
Prospec"sproductsarefurnishedforLABORATORYRESEARCHUSEONLY.Theproductmaynotbeusedasdrugs,agriculturalorpesticidalproducts,foodadditivesorhouseholdchemicals.
SafetyDataSheet
References
1.Title:CandidaalbicansabrogatestheexpressionofIFN-G-inducIBLeprotein-10inhumankeratinocytes.Publication:Articlefirstpublishedonline:18JUL2008DOI:10.1111/j.1574-695X.2008.00457.xLink:http://onlinelibrary.wiley.com/doi/10.1111/j.1574-695X.2008.00457.x/full
2.Title:AugmentsCytokine-InducedNitricOxideProductioninHumanAlveolarEpithelialCellsPublications:Antimicrobialagentsandchemotherapy50.1(2006):396-398Link:http://aac.asm.org/content/50/1/396.full
3.Title:SelectiveActivationofHumanDendriticCellsbyOM-85throughaNF-kBandMAPKDependentPathwayPublications:PloSone8.12(2013):e82867Link:http://journals.plos.org/plosone/article?id=10.1371/journal.pone.0082867#pone-0082867-g005
4.Title:ANewStrategytoInduceEffectiveAntitumourResponseInVitroandInVivoPublications:Scandinavianjournalofimmunology68.3(2008):287-296Link:http://onlinelibrary.wiley.com/doi/10.1111/j.1365-3083.2008.02140.x/full
5.Title:CaptureofTumorCellMembranesbyTrogocytosisFacilitatesDetectionandIsolationofTumor-SpecificFunctionalCTLsPublication:Cancerresearch68.6(2008):2006-2013.Link:http://cancerres.aacrjournals.org/content/68/6/2006.full
6.Title:Curcumininducesmaturation-arresteddendriticcellsthatexpandregulatoryTcellsinvitroandinvivoPublication:Clinical&ExperimentalImmunology162.3(2010):460-473.Link:http://onlinelibrary.wiley.com/doi/10.1111/j.1365-2249.2010.04232.x/full