
Cataloguenumber
CYT-204
Synonyms
LeukocyteIFN,BcellIFN,TypeIIFN,IFNA2,IFN-a2a.
Introduction
IFN-alphaisproducedbymacrophagesandhasantiviralactivities.IFNstimulatestheproductionoftwoenzymes:proteinkinaseandanoligoadenylatesynthetase.
Description
IFNAlphaHuman2aRecombinantproducedinE.Coliisasingle,non-glycosylated,polypeptidechaincontaining165aminoacidsandhavingamolecularmassof19241Dalton.ThedifferencebetweenIFNA2AandIFNA2Bisintheaminoacidpresentatposition23.IFN-alpha2ahasalysineatthatposition23whileIFN-alpha2bhasarginine.TheIFNA2Agenewasobtainedfromhumanleukocytes.TheIFNA2Aispurifiedbyproprietarychromatographictechniques.
Source
PhysicalAppearance
Formulation
Solubility
ItisrecommendedtoreconstitutethelyophilizedIFNA2Ainsterile18MΩ-cmH2Onotlessthan100µg/ml,whichcanthenbefurtherdilutedtootheraqueoussolutions.
StABIlity
LyophilizedIFNA-2Aalthoughstableatroomtemperaturefor3weeks,shouldbestoreddesiccatedbelow-18°C.UponreconstitutionIFN-alpha2ashouldbestoredat4°Cbetween2-7daysandforfutureusebelow-18°C.Forlongtermstorageitisrecommendedtoaddacarrierprotein(0.1%HSAorBSA).Pleasepreventfreeze-thawcycles.
Purity
Aminoacidsequence
CDLPQTHSLGSRRTLMLLAQMRKISLFSCLKDRHDFGFPQEEFGNQFQKAETIPVLHEMIQQIFNLFSTKDSSAAWDETLLDKFYTELYQQLNDLEACVIQGVGVTETPLMKEDSILAVRKYFQRITLYLKEKKYSPCAWEVVRAEIMRSFSLSTNLQESLRSKE.
N-terminalmethioninehasbeencompletelyremovedenzymatically.
BIOLOGicalActivity
Proteincontent
References
1.Title:Genderspecificityofalteredhumanimmunecytokineprofilesinaging.Publication:PublishedonlinebeforeprintMay7,2010,doi:10.1096/fj.10-160911September2010TheFASEBJournalvol.24no.93580-3589Link:http://www.fasebj.org/content/24/9/3580.full
SafetyDataSheet
SDS