
Cataloguenumber
CYT-1027
Synonyms
Follistatin-relatedprotein1,Follistatin-likeprotein1,FSTL1,FRP,FollistatinLike1,FSL1,MIR198.
Introduction
FSTL1proteinresemblesfollistatin,anactivin-bindingprotein.FSTL1isanautoantigenassociatedwithrheumatoidarthritisanditholdsanFSsection,afollistatin-likesequencehaving10conservedcysteineresidues.
Description
FSTL1HumanRecombinantproducedinHEK293cellsisasingle,glycosylatedpolypeptidechain(a.a21-308)containing296aminoacidsincludinga8a.aC-terminalHistag.Thetotalmolecularmassis33.8kDa(calculated).
Source
HEK293cells.
PhysicalAppearance
Formulation
FSTL1filtered(0.4µm)andlyophilizedfrom0.5mg/mlinphosphatebufferedsalineand5%(w/v)trehalose,pH7.4.
Solubility
Itisrecommendedtoadd200µldeionizedwatertoprepareaworkingstocksolutionofapproximately0.5mg/mlandletthelyophilizedpelletdissolvecompletely.FSTL1isnotsterile!Pleasefiltertheproductbyanappropriatesterilefilterbeforeusingitinthecellculture.
StABIlity
Storelyophilizedproteinat-20°C.Aliquottheproductafterreconstitutiontoavoidrepeatedfreezing/thawingcycles.Reconstitutedproteincanbestoredat4°Cforalimitedperiodoftime;itdoesnotshowanychangeafteroneweekat4°C.
Purity
Greaterthan95.0%asdeterminedbySDS-PAGE.
Aminoacidsequence
EEELRSKSKICANVFCGAGRECAVTEKGEPTCLCIEQCKPHKRPVCGSNGKTYLNHCELHRDACLTGSKIQVDYDGHCKEKKSVSPSASPVVCYQSNRDELRRRIIQWLEAEIIPDGWFSKGSNYSEILDKYFKNFDNGDSRLDSSEFLKFVEQNETAINITTYPDQENNKLLRGLCVDALIELSDENADWKLSFQEFLKCLNPSFNPPEKKCALEDETYADGAETEVDCNRCVCACGNWVCTAMTCDGKNQKGAQTQTEEEMTRYVQELQKHQETAEKTKRVSTKEIHHHHHHHH.
SafetyDataSheet
SDS
Usage
Prospec"sproductsarefurnishedforLABORATORYRESEARCHUSEONLY.Theymaynotbeusedasdrugs,agriculturalorpesticidalproducts,foodadditivesorhouseholdchemicals.