Cataloguenumber
CYT-232
Synonyms
Introduction
Follistatinisasingle-chaingonadalproteinthatspecificallyinhibitsfollicle-stimulatinghormonerelease.ThesingleFSTgeneencodestwoisoforms,FST317andFST344containing317and344aminoacidsrespectively,resultingfromalternativesplicingoftheprecursormRNA.Inastudyinwhich37candidategenesweretestedforlinkageandassociationwithpolycysticovarysyndrome(PCOS)orhyperandrogenemiain150families,evidencewasfoundforlinkagebetweenPCOSandfollistatin.FollistatinfunctionsasanACTVantagoNIST.specificinhibitorofthebiosynthesisandsecretionofpituitaryfolliclestimulatinghormone(fsh).
Description
FollistatinHumanRecombinantproducedinE.Coliisasingle,non-glycosylatedpolypeptidechaincontaining288aminoacidsandhavingatotalmolecularmassof31.5kDa.TheFSTispurifiedbyproprietarychromatographictechniques.
Source
EscherichiaColi.
PhysicalAppearance
SterileFilteredWhitelyophilized(freeze-dried)powder.
Formulation
Lyophilizedfromaconcentrated(1mg/ml)solutioncontainingnoadditives.
Solubility
ItisrecommendedtoreconstitutethelyophilizedFollistatininsterile18MΩ-cmH2Onotlessthan100µg/ml,whichcanthenbefurtherdilutedtootheraqueoussolutions.
LyophilizedFollistatinalthoughstableatroomtemperaturefor3weeks,shouldbestoreddesiccatedbelow-18°C.UponreconstitutionFSTshouldbestoredat4°Cbetween2-7daysandforfutureusebelow-18°C.Forlongtermstorageitisrecommendedtoaddacarrierprotein(0.1%HSAorBSA).Pleasepreventfreeze-thawcycles.
Purity
Greaterthan95.0%asdeterminedby:(a)AnalysisbyRP-HPLC.(b)AnalysisbySDS-PAGE.
Aminoacidsequence
ThesequenceofthefirstfiveN-terminalaminoacidswasdeterminedandwasfoundtobeGly-Asn-Cys-Trp-Leu.
TheactivityisdeterminedbytheabilitytoneutralizeACTVinhibitoryeffectofmouseMPC-11cells.TheexpectedED50is100-400ng/ml,correspondingtoaSpecificActivityof2,500-10,000units/mginthepresenceof7.5ng/mlACTVA.
SafetyDataSheet
Usage
Prospec"sproductsarefurnishedforLABORATORYRESEARCHUSEONLY.Theymaynotbeusedasdrugs,agriculturalorpesticidalproducts,foodadditivesorhouseholdchemicals.