
Cataloguenumber
CYT-340
Synonyms
Introduction
Description
Flt3-LigandMouseRecombinantproducedinE.Coliisanon-glycosylated,polypeptidechaincontaining163aminoacidsandhavingamolecularmassof18.6kDa.Flt3-Ligandispurifiedbyproprietarychromatographictechniques.
Source
PhysicalAppearance
Formulation
TheFLT3LMouseproteinwaslyophilizedfromsterilefilteredsolutioncontaining10mMsodiumphosphate,pH7.5.
Solubility
ItisrecommendedtoreconstitutethelyophilizedFlt3lMouserecombinantinsterile18MΩ-cmH2Oataconcentrationof100µg/ml,whichcanthenbefurtherdilutedtootheraqueoussolutions.
StABIlity
LyophilizedFlt3-Ligandalthoughstableatroomtemperaturefor3weeks,shouldbestoreddesiccatedbelow-18°C.UponreconstitutionFlt-3Ligandshouldbestoredat4°Cbetween2-7daysandforfutureusebelow-18°C.Forlongtermstorageitisrecommendedtoaddacarrierprotein(0.1%HSAorBSA).Pleasepreventfreeze-thawcycles.
Purity
Greaterthan95.0%asdeterminedbySDS-PAGE.
Aminoacidsequence
BIOLOGicalActivity
TheED50,calculatedbythedose-dependentproliferationofmouseAML5cellsis1.66ng/ml,correspondingtoaspecificactivityof6.0x105units/mg.
SafetyDataSheet
SDS