ApolipoproteinA-I,Apo-AI,ApoA-I,APOA1,MGC117399.
APOA1(ApolipoproteinA-1)isahumanproteinwithaspecificroleinlipidmetabolismbeingthemainproteincomponentofHDLintheplasma.APOA1promotescholesteroleffluxfromtissuestotheliverforexcretion.Fur
Thermore,APOA1isacofactorforLCAT,whichisrespons
IBLefortheformationofmostplasmacholesterylesters.Inaddition,APOA1activatesspermatozoamotilityaspartoftheSPAPcomplex.TheAPOA1geneisstronglylinkedwithtwootherapolipoproteingenesonchromosome11.DefectsintheAPOA1genearelinkedtoHDLdeficiencyincludingTangierdisease,andwithsystemicnon-neuropathicamyloidosis.HighlevelsofAPOA1arelinkedtothemanifestationofasthmaandatopy.
ApolipoproteinA-IHumanRecombinantproducedinE.coliisasingle,non-glycosylatedpolypeptidechaincontaining243aminoacidsandhavingamolecularmassof28.1kDa.
TheAPOA1ispurifiedbyproprietarychromatographictechniques.
EscherichiaColi.
SterileFilteredWhitelyophilized(freeze-dried)powder.
TheAPOA1proteinwaslyophilizedfroma0.2µmfilteredconcentratedsolutioninPBS,pH7.4.
ItisrecommendedtoreconstitutethelyophilizedAPOA1insterile18MΩ-cmH2Onotlessthan100µg/ml,whichcanthenbefurtherdilutedtootheraqueoussolutions.
LyophilizedApolipoproteinA-Ialthoughstableatroomtemperaturefor3weeks,shouldbestoreddesiccatedbelow-18°C.UponreconstitutionAPOA1shouldbestoredat4°Cbetween2-7daysandforfutureusebelow-18°C.Forlongtermstorageitisrecommendedtoaddacarrierprotein(0.1%HSAorBSA).Pleasepreventfreeze-thawcycles.
Greaterthan98.0%asdeterminedby:(a)AnalysisbyRP-HPLC.(b)AnalysisbySDS-PAGE.
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Prospec"sproductsarefurnishedforLABORATORYRESEARCHUSEONLY.Theproductmaynotbeusedasdrugs,agriculturalorpesticidalproducts,foodadditivesorhouseholdchemicals.