Cataloguenumber
CYT-741
Synonyms
IFN-lamBDa-2,Interleukin-28A,IL-28A,Ifnl2,Il28a,EG330496.
Introduction
IL-28AisdistantlyrelatedtotheIL-10family.ExpressionofIL-28AisinducedbyviralinfectionwhichinteractswithaheterodimericclassIIcytokinereceptorthatconsistsofinterleukin10receptor,beta(IL10RB)andinterleukin28receptor,alpha.IL-28AexhibitscommonfeatureswithtypeIIFNssuchasantiviralactivity,antiproliferativeactivityandinvivoantitumouractivity.IL-28AactssimilarlytoIFNs,butislesseffectivegenerallyandhasactivityinamorelimitedrangeofcelllines.IFN-ambda1,IFN-lambda2andIFN-lambda3arecloselypositionedgenesonhumanchromosome19.IL-28AinducesELR(-)CXCchemokinemRNAinhumanperipheralbloodmononuclearcells,inanIFN-gamma-independentmanner.IL-28AisabletogeneratetolerogenicDCs,anactivitythatcouldthwartIFN-betafunctions.IL-28Aproducedinresponsetoviralinfection,activatesbothmonocytesandmacrophagesproducingarestrictedpanelofcytokinesandthereforeisanimportantfactorinactivatinginnateimmuneresponsesatthesiteofviralinfection.
Description
RecombinantMouseInterleukin-28AproducedinE.Coliisasingle,non-glycosylatedpolypeptidechaincontaining174aminoacidsandhavingatotalmolecularmassof19.7kDa.TheIL-28Aispurifiedbyproprietarychromatographictechniques.
Source
EscherichiaColi.
PhysicalAppearance
SterileFilteredWhitelyophilized(freeze-dried)powder.
Formulation
TheMouseIL-28Aislyophilizedfroma0.2µmfilteredconcentratedsolutioninPBS,pH7.4and5%trehalose.
Solubility
ItisrecommendedtoreconstitutethelyophilizedIL-28Ainsterile18MΩ-cmH2Onotlessthan100µg/ml,whichcanthenbefurtherdilutedtootheraqueoussolutions.
LyophilizedIFN-Lambda2althoughstableatroomtemperaturefor3weeks,shouldbestoreddesiccatedbelow-18°C.UponreconstitutionIFN-Lambda2Recombinantshouldbestoredat4°Cbetween2-7daysandforfutureusebelow-18°C.Forlongtermstorageitisrecommendedtoaddacarrierprotein(0.1%HSAorBSA).Pleasepreventfreeze-thawcycles.
Aminoacidsequence
DPVPRATRLPVEAKDCHIAQFKSLSPKELQAFKKAKDAIEKRLLEKDMRCSSHLISRAWDLKQLQVQERPKALQAEVALTLKVWENMTDSALATILGQPLHTLSHIHSQLQTCTQLQATAEPKPPSRRLSRWLHRLQEAQSKETPGCLEDSVTSNLFRLLTRDLKCVASGDQCV.
Purity
Greaterthan95%asdeterminedby:(a)AnalysisbyRP-HPLC.(b)AnalysisbySDS-PAGE.
TheED50asdeterminedbyananti-viralassayusinghumanHepG2cellsinfectedwithencephalomyocarditis(EMC)virusislessthan3ng/ml,correspondingtoaspecificactivityof>3.3×105IU/mg.
SafetyDataSheet
Usage
Prospec"sproductsarefurnishedforLABORATORYRESEARCHUSEONLY.Theproductmaynotbeusedasdrugs,agriculturalorpesticidalproducts,foodadditivesorhouseholdchemicals.