T-cellgrowthfactor(TCGF),Lymphokine,IL-2.
IL2isasecretedcytokinethatisimportantfortheproliferationofTandBlymphocytes.Thereceptorofthiscytokineisaheterotrimericproteincomplexwhosegammachainisalsosharedbyinterleukin4(IL4)andinterleukin7(IL7).Theexpressionofthisgeneinmaturethymocytesismonoallelic,whichrepresentsanunusualregulatorymodeforcontrollingthepreciseexpressionofasinglegene.Thetargeteddisruptionofasimilargeneinmiceleadstoulcerativecolitis-likedisease,whichsuggestsanessentialroleofthisgeneintheimmuneresponsetoantigenicstimuli.
Interleukin-2HumanRecombinantproducedinE.Coliisasingle,non-glycosylatedpolypeptidechaincontaining133aminoacidsandhavingamolecularmassof15.4kDa.
TheIL-2ispurifiedbyproprietarychromatographictechniques.
EscherichiaColi.
SterileFilteredWhitelyophilized(freeze-dried)powder.
Theproteinwaslyophilizedfroma0.2μmfilteredconcentratedsolution(1.0mg/ml)inPBS,pH5.0.
ItisrecommendedtoreconstitutethelyophilizedInterleukin-2insteriledistilledwaternotlessthan100µg/ml,whichcanthenbefurtherdilutedtootheraqueoussolutions.
LyophilizedInterleukin-2althoughstableatroomtemperaturefor3weeks,shouldbestoreddesiccatedbelow-18°C.UponreconstitutionIL2shouldbestoredat4°Cbetween2-7daysandforfutureusebelow-18°C.Forlongtermstorageitisrecommendedtoaddacarrierprotein(0.1%HSAorBSA).Pleasepreventfreeze-thawcycles.
Greaterthan97.0%asdeterminedby:(a)AnalysisbyRP-HPLC.(b)AnalysisbySDS-PAGE.
APTSSSTKKTQLQLEHLLLDLQMILNGINNYKNPKLTRMLTFKFYMPKKATELKHLQCLEEELKPLEEVLNLAQSKNFHLRPRDLISNINVIVLELKGSETTFMCEYADETATIVEFLNRWITFCQSIISTLT.
TheED50asdeterminedbythedose-dependentstimulationofmurineCTLL-2cellsislessthan0.1ng/ml,correspondingtoaSpecificActivityof1x107IU/mg.
1.
Title:CD8KineticallyPromotesLigandBindingtotheT-CellAntigenReceptor.
Publication:BiophysicalJournalVolume89September20052121–2133.
Link:http://www.ncbi.nlm.nih.gov/pmc/articles/PMC1366714/pdf/2121.pdf
2.
Title:DetectionofexpressedIL-32inhumanstomachcancerusing
ELISAandimmunostaining
Publication:JMicrobiolBiotechnol
18.9(2008):1606-1612.Link:http://www.jmb.or.kr/submission/Journal/018/JMB018-09-19.pdf3.Title:Conservationofcytokine-mediatedresponsesininnateimmunity:aflowcytometricstudyinvestigatingtheeffectsofhumanproinflammatorycytokinesonphagocytosisintheearthwormEiseniahortensis
Publication:InvertebrateSurvivalJournal5(2008):124-134.
Link:http://www.isj.unimo.it/articoli/ISJ175.pdf
Prospec"sproductsarefurnishedforLABORATORYRESEARCHUSEONLY.Theproductmaynotbeusedasdrugs,agriculturalorpesticidalproducts,foodadditivesorhouseholdchemicals.