
Cataloguenumber
CYT-1122
Synonyms
ImmuneIFN,typeIIIFN,TcellIFN,MAF,IFNG,IFG,IFI,IFN-gamma.
Introduction
IFN-gammaproducedbylymphocytesactivatedbyspecificantigens/mitogens.IFN-gamma,ontopofhavingantiviralactivity,hasimportantimmunoregulatoryfunctions,itisapotentactivatorofmacrophages,andhasantiproliferativeeffectsontransformedcellsanditcanpotentiatetheantiviralandantitumoreffectsofthetypeIinterferons.
Description
Interferon-gammaRhesusMacaqueRecombinantproducedinE.Coliisanon-glycosylatedpolypeptidechaincontaining142aminoacidandhavingamolecularmassofapproximately16.8kDa.IFNGispurifiedbyproprietarychromatographictechniques.
Source
EscherichiaColi.
PhysicalAppearance
SterileFilteredWhitelyophilized(freeze-dried)powder.
Formulation
Lyophilizedfroma0.2μmfilteredconcentratedsolutioninPBS,pH7.4.
Solubility
ItisrecommendedtoreconstitutethelyophilizedInterferon-gammaRhesusMacaqueinsterile18MΩ-cmH2Onotlessthan100µg/ml,whichcanthenbefurtherdilutedtootheraqueoussolutions.
StABIlity
LyophilizedIFNGalthoughstableatroomtemperaturefor3weeks,shouldbestoreddesiccatedbelow-18°C.UponreconstitutionInterferon-gammaRhesusMacaqueshouldbestoredat4°Cbetween2-7daysandforfutureusebelow-18°C.Pleasepreventfreeze-thawcycles.
Purity
Greaterthan97.0%asdeterminedby:(a)AnalysisbyRP-HPLC.(b)AnalysisbySDS-PAGE.
Aminoacidsequence
QDPYVKEAENLKKYFNAGDPDVADNGTLFLDILRNWKEESDRKIMQSQIVSFYFKLFKNFKDDQRIQKSVETIKEDINVKFFNSNKKKRDDFEKLTNYSVTDSNVQRKAVHELIQVMAELSPAAKIGKRKRSQMFRGRRASQ.
BIOLOGicalActivity
TheED50asdeterminedbyananti-viralassayusinghumanHeLacellsinfectedwithencephalomyocarditis(EMC)virusis<20.0ng/ml,correspondingtoaspecificactivityof>5.0×104IU/mg.
SafetyDataSheet
SDS