
Cataloguenumber
CYT-1128
Synonyms
Cardiotrophin-2,CT-2,Neuropoietin,Np,Ctf2,Gm494.
Introduction
CTF2P,akaNeuropoietinisapartoftheIL-6familyofcytokines.CTF2Pistheoutcomeofageneduplicationeventinvolvingcardiotrophin-1(CT-1)andithelpstodefineasubfamilywithintheIL-6familythatincludesCT-1,CLCandCTNF.CTF2PIncreasestheplateletcountassociatedwithsplenomegalyandtakespartinneuronalprecursordevelopmentandmaturation.
Description
NeuropoietinMouseRecombinantproducedinE.Coliisanon-glycosylatedpolypeptidechaincontaining182aminoacidsandhavingamolecularmassofapproximately19.7kDa.CTF2Pispurifiedbyproprietarychromatographictechniques.
Source
EscherichiaColi.
PhysicalAppearance
SterileFilteredWhitelyophilized(freeze-dried)powder.
Formulation
Lyophilizedfroma0.2μmfilteredconcentratedsolutionin20mMTris-HCl,pH8.0,0.5mMDTTand500mMNaCl.
Solubility
ItisrecommendedtoreconstitutethelyophilizedNeuropoietininsterilePBSnotlessthan100µg/ml,whichcanthenbefurtherdilutedtootheraqueoussolutions.
StABIlity
LyophilizedCTF2Palthoughstableatroomtemperaturefor3weeks,shouldbestoreddesiccatedbelow-18°C.UponreconstitutionNeuropoietinshouldbestoredat4°Cbetween2-7daysandforfutureusebelow-18°C.
Pleasepreventfreeze-thawcycles.
Purity
Greaterthan98.0%asdeterminedby:(a)AnalysisbyRP-HPLC.(b)AnalysisbySDS-PAGE.
Aminoacidsequence
APISPSEPIGQAYSLALYMQKNTSALLQTYLQHQGSPFSDPGFSAPELQLSTLPSAAVSFKTWHAMEDAERLSRAQGAFLALTQHLQLVGDDQSYLNPGSPILLAQLGAARLRAQGLLGNMAAIMTALGLPIPPEEDTLGFVPFGASAFERKCRGYIVTREYGHWTDRAVRDLALLKAKYSA.
BIOLOGicalActivity
TheED50asdeterminedbyacellproliferationassayusinghumanTF-1cellsis<200ng/ml,correspondingtoaspecificactivityof>5000IU/mg.
SafetyDataSheet
SDS