
Cataloguenumber
CYT-1118
Synonyms
NPPB,NatriureticPeptidePrecursorB,BNP,B-typeNatriureticPeptide.
Introduction
NatriureticPeptidePrecursorBactsasacardiachormonewithavarietyofBIOLOGicalactionsincludingnatriuresis,vasorelaxation,diuresisandinhibitionofreninandaldosteronesecretion.BNPplaysakeyroleincardiovascularhomeostasis.BNPalsohelpsrestorethebody"ssaltandwaterbalanceandimprovesheartfunction.
Description
NT-Pro-B-typeNatriureticProteinHumanRecombinantproducedinE.Coliisanon-glycosylatedpolypeptidechaincontaining76aminoacidandhavingamolecularmassofapproximately8.5kDa.
NT-proBNPispurifiedbyproprietarychromatographictechniques.
Source
EscherichiaColi.
PhysicalAppearance
SterileFilteredWhitelyophilized(freeze-dried)powder.
Formulation
Lyophilizedfroma0.2μmfilteredconcentratedsolutionin20mMTris-HCl,pH8.0and150mMNaCl.
Solubility
ItisrecommendedtoreconstitutethelyophilizedNT-Pro-B-typeNatriureticProteininsterile18MΩ-cmH2Onotlessthan100µg/ml,whichcanthenbefurtherdilutedtootheraqueoussolutions.
StABIlity
LyophilizedNT-proBNPalthoughstableatroomtemperaturefor3weeks,shouldbestoreddesiccatedbelow-18°C.UponreconstitutionNT-Pro-B-typeNatriureticProteinshouldbestoredat4°Cbetween2-7daysandforfutureusebelow-18°C.
Pleasepreventfreeze-thawcycles.
Purity
Greaterthan98.0%asdeterminedby:(a)AnalysisbyRP-HPLC.(b)AnalysisbySDS-PAGE.
Aminoacidsequence
HPLGSPGSASDLETSGLQEQRNHLQGKLSELQVEQTSLEPLQESPRPTGVWKSREVATEGIRGHRKMVLYTLRAPR.
SafetyDataSheet
SDS