
Cataloguenumber
CYT-1071
Synonyms
BoneMarrowStromalCellAntigen1,ADP-RibosylCyclase2,BoneMarrowStromalAntigen1,CyclicADP-RiboseHydrolase2,NAD(+)Nucleosidase,CADPrHydrolase2,ADP-RibosylCyclase/CyclicADP-RiboseHydrolase2,CD157Antigen,EC3.2.2.6,CD157,BST-1,ADP-ribosylcyclase/cyclicADP-ribosehydrolase2,ADP-ribosylcyclase2,Bonemarrowstromalantigen1,CyclicADP-ribosehydrolase2,cADPrhydrolase2.
Introduction
BST1(BoneMarrowStromalCellAntigen1),isaGPI(glycosylphosphatidylinositol)anchoredmembraneproteinwhichispartoftheCD38family.BST1wasinitiallyrecognizedasabonemarrowstromalcellmolecule.BST1isanectoenzymesharingmorethanafewfeatureswithADP-ribosylcyclaseCD38.BST1togetherwithCD38,exhibitbothDP-ribosylcyclaseandcyclincADPribosehydrolaseactivities.BST1participatesinrheumatoidarthritisduetoitsenhancedexpressioninRA-derivedbonemarrowstromalcelllines.Moreover,BST1isexpressedbycellsofthemyeloidlineageandcouldperformasareceptorwithasignaltransductioncapABIlity.
Description
BST1producedinSf9Baculoviruscellsisasingle,glycosylatedpolypeptidechaincontaining267aminoacids(33-293a.a.)andhavingamolecularmassof30.5kDa.(MolecularsizeonSDS-PAGEwillappearatapproximately40-57kDa).BST1isexpressedwitha6aminoacidHistagatC-Terminusandpurifiedbyproprietarychromatographictechniques.
Source
Sf9,Baculoviruscells.
PhysicalAppearance
Sterilefilteredcolorlesssolution.
Formulation
BST1proteinsolution(0.5mg/ml)contains10%glycerol&PhosphateBufferedSaline(pH7.4).
Stability
Purity
Greaterthan95.0%asdeterminedbySDS-PAGE.
Aminoacidsequence
RWRGEGTSAHLRDIFLGRCAEYRALLSPEQRNKNCTAIWEAFKVALDKDPCSVLPSDYDLFINLSRHSIPRDKSLFWENSHLLVNSFADNTRRFMPLSDVLYGRVADFLSWCRQKNDSGLDYQSCPTSEDCENNPVDSFWKRASIQYSKDSSGVIHVMLNGSEPTGAYPIKGFFADYEIPNLQKEKITRIEIWVMHEIGGPNVESCGEGSMKVLEKRLKDMGFQYSCINDYRPVKLLQCVDHSTHPDCALKSAAAATQRKAHHHHHH.
SafetyDataSheet
SDS