Cataloguenumber
CYT-380
Synonyms
BMPR-1A,BMP-R1A,BMPR1A,BMR1A,CD292,CD-292,Serine/threonine-proteinkinasereceptorR5,SKR5,ALK-3,ACVRLK3,EC2.7.11.30,CD292antigen.
Introduction
Thebonemorphogeneticprotein(BMP)receptorsareafamilyoftransmembraneserine/threoninekinasesthatincludethetypeIreceptorsBMPR1AandBMPR1BandthetypeIIreceptorBMPR2.Thesereceptorsarealsocloselyrelatedtothereceptors,ACVR1andACVR2.TheligandsofthesereceptorsaremembersoftheTGF-betasuperfamily.TGF-betastransducetheirsignalsthroughtheformationofheteromericcomplexeswith2differenttypesofserine(threonine)kinasereceptors:typeIreceptorsofabout50-55kDandtypeIIreceptorsofabout70-80kD.TypeIIreceptorsbindligandsintheabsenceoftypeIreceptors,buttheyrequiretheirrespectivetypeIreceptorsforsignaling,whereastypeIreceptorsrequiretheirrespectivetypeIIreceptorsforligandbinding.
Description
BMPR1AHumanRecombinantextracellulardomainproducedinbaculovirusisamonomeric,glycosylated,Polypeptidechainfusedwith6xHistagatC-terminusandhavingamolecularmassof23kDa.TheBMR1Aispurifiedbyproprietarychromatographictechniques.
Source
InsectCells.
PhysicalAppearance
SterileFilteredWhitelyophilized(freeze-dried)powder.
Formulation
CD292waslyophilizedfromaconcentrated(1mg/ml)sterilesolutioncontaining1XPBS.
Solubility
ItisrecommendedtoreconstitutethelyophilizedALK-3insterilePBSnotlessthan100µg/ml,whichcanthenbefurtherdilutedtootheraqueoussolutions.
LyophilizedBoneMorphogeneticProteinReceptor1Aalthoughstableatroomtemperaturefor3weeks,shouldbestoreddesiccatedbelow-18°C.UponreconstitutionBMPR1Ashouldbestoredat4°Cbetween2-7daysandforfutureusebelow-18°C.Forlongtermstorageitisrecommendedtoaddacarrierprotein(0.1%HSAorBSA).Pleasepreventfreeze-thawcycles.
Purity
Greaterthan90.0%asdeterminedby(a)AnalysisbyRP-HPLC.(b)AnalysisbySDS-PAGE.
MeasuredbyitsabilitytoinhibitrecombinanthumanBMP-2inducedalkalinephosphataseproductionbyC2C12myogeniccells.TheED50forthiseffectistypically1-3µg/mlinthepresenceof500ng/mlofrecombinanthumanBMP-2correspondingtoaSpecificActivityof2,000units/mg.
SafetyDataSheet
Usage
Prospec"sproductsarefurnishedforLABORATORYRESEARCHUSEONLY.Theproductmaynotbeusedasdrugs,agriculturalorpesticidalproducts,foodadditivesorhouseholdchemicals.