
Cataloguenumber
CYT-1093
Synonyms
BMP4,ZYME,BMP2B,BMP2B1.
Introduction
Theproteinencodedbythisgeneisamemberofthebonemorphogeneticproteinfamilywhichispartofthetransforminggrowthfactor-betasuperfamily.Thesuperfamilyincludeslargefamiliesofgrowthanddifferentiationfactors.BonemorphogeneticproteinswereoriginallyidentifiedbyanABIlityofdemineralizedboneextracttoinduceendochondralosteogenesisinvivoinanextraskeletalsite.Thisparticularfamilymemberplaysanimportantroleintheonsetofendochondralboneformationinhumans,andareductioninexpressionhasbeenassociatedwithavarietyofbonediseases,includingtheheritabledisorderFibrodysplasiaOssificansProgressiva.Alternativesplicinginthe5"untranslatedregionofthisgenehasbeendescribedandthreevariantsaredescribed,allencodinganidenticalprotein.
Description
BoneMorphogeneticprotein-4ActiveHumanRecombinantproducedinCHOcellsisaglycosylatedhomodimerchaincontaining2x116aminoacidsandhavingatotalmolecularmassof26.2kDa.
BMP4ispurifiedbyproprietarychromatographictechniques.
Source
CHOcells.
PhysicalAppearance
Formulation
Theproteinwaslyophilizedfromasterile(0.2µm)filteredsolutioncontaining0.1%TrifluoroaceticAcid(TFA).
Solubility
ItisrecommendedtoreconstitutethelyophilizedBMP4insterile18MΩ-cmH2Onotlessthan100µg/ml,whichcanthenbefurtherdilutedtootheraqueoussolutions.
Stability
LyophilizedBMP4althoughstableatroomtemperaturefor3weeks,shouldbestoreddesiccatedbelow-18°C.UponreconstitutionBMP4shouldbestoredat4°Cbetween2-7daysandforfutureusebelow-18°C.
Pleasepreventfreeze-thawcycles.
Aminoacidsequence
SPKHHSQRARKKNKNCRRHSLYVDFSDVGWNDWIVAPPGYQAFYCHGDCPFPLADHLNSTNHAIVQTLVNSVNSSIPKACCVPTELSAISMLYLDEYDKVVLKNYQEMVVEGCGCR.
Purity
Greaterthan95.0%asdeterminedbySDS-PAGE.
BIOLOGicalActivity
TheED50,ascalculatedbyAlkalinephosphataseactivityinducedinATDC-5cellsis11.2ng/mlcorrespondingtoaspecificactivitywhichis8.9x10^4units/mg.
SafetyDataSheet
SDS