
Cataloguenumber
CYT-1042
Synonyms
APOB,APO-B,ApolipoproteinB.
Introduction
ApoBisthemainapolipoproteinofLDL,IDL,VLDLandchylomicronsparticlesthatservesasthecarrieroflipids(fatmolecules),aswellascholesterol,inthewatersurroundingthecellswithineverytissueacrossthebody.ThoughallthefunctionalaspectsofApolipoproteinBwithintheLDLandotherelementsareconsideredtoberatheruncertain,itservesasthekeyorganizingproteinofallothercarriersoflipids.acrossLDLmembranes,apolipoproteinBalsoservesasaligandforLDLreceptorsinmanycellsacrossthebody,meaning,itshowsthatlipidcarriersthataresettocrossintocellswithApolipoproteinBreceptors,thisisthewaythatlipidsaretransportedwithinandintocells.
Description
HumanAPOBproducedfromHumanplasmahavingamolecularmassof550kDa.
Source
HumanPlasma.
PhysicalAppearance
SterileFilteredlyophilized(freeze-dried)powder.
Formulation
Theproteinwaslyophilizedfroma0.5mg/mlsolutionof50mMNa2CO3pH-10containing50mMNaCland10mMsodiumdeoxycholate.
Solubility
ItisrecommendedtoreconstitutethelyophilizedAPOBinsterile18M-cmH2Onotlessthan100µg/ml,whichcanthenbefurtherdilutedtootheraqueoussolutions.
StABIlity
LyophilizedAPOBalthoughstableatroomtemperaturefor3weeks,shouldbestoreddesiccatedbelow-18°C.Uponreconstitution,APOBshouldbestoredat4°Cbetween2-7daysandforfutureusebelow-18°C.Forlong-termstorage,itisrecommendedtoaddacarrierprotein(0.1%HSAorBSA).Pleasepreventfreeze-thawcycles.
Purity
Greaterthan95.0%.
HumanVirusTest
StartingmaterialdonorhasbeentestedandcertifiednegativeforantibodiestoHIV-1,HIV-2,HCV,HBSAG.
SafetyDataSheet
SDS